| 货号 | 2601T |
| 反应种属 | Human/Mouse/GuineaPig |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-PAK1 (Thr423)/PAK2 (Thr402) Antibody detects endogenous PAK1, PAK2 and PAK3 only when phosphorylated at Thr423, Thr402 and Thr421, respectively. The antibody does not cross-react with phosphorylated PAK4, PAK5 or PAK6. The antibody does cross-react with phospho-Mst1 (Thr183) or phospho-Mst2 (Thr180). |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The p21-activated kinase (PAK) family of serine/threonine kinases is engaged in multiple cellular processes, including cytoskeletal reorganization, MAPK signaling, apoptotic signaling, control of phagocyte NADPH oxidase, and growth factor-induced neurite outgrowth (1,2). Several mechanisms that induce PAK activity have been reported. Binding of Rac/Cdc42 to the CRIB (or PBD) domain near the amino terminus of PAK causes autophosphorylation and conformational changes in PAK (1). Phosphorylation of PAK1 at Thr423 by PDK induces activation of PAK1 (3). Several autophosphorylation sites have been identified, including Ser199 and Ser204 of PAK1 and Ser192 and Ser197 of PAK2 (4,5). Because the autophosphorylation sites are located in the amino-terminal inhibitory domain, it has been hypothesized that modification in this region prevents the kinase from reverting to an inactive conformation (6). Research indicates that phosphorylation at Ser144 of PAK1 or Ser139 of PAK3 (located in the kinase inhibitory domain) affects kinase activity (7). Phosphorylation at Ser21 of PAK1 or Ser20 of PAK2 regulates binding with the adaptor protein Nck (8). PAK4, PAK5, and PAK6 have lower sequence similarity with PAK1-3 in the amino-terminal regulatory region (9). Phosphorylation at Ser474 of PAK4, a site analogous to Thr423 of PAK1, may play a pivotal role in regulating the activity and function of PAK4 (10). |
| 存放说明 | -20C |
| 计算分子量 | 61 to 67 (PAK2), 68 to 74 (PAK1/3) |
| 参考文献 | 1 . Knaus, U.G. and Bokoch, G.M. (1998) Int. J. Biochem. Cell Biol. 30, 857-862. 2 . Daniels, R.H. et al. (1998) EMBO J. 17, 754-764. 3 . King, C.C. et al. (2000) J. Biol. Chem. 275, 41201-41209. 4 . Manser, E. et al. (1997) Mol. Cell. Biol. 17, 1129-1143. 5 . Gatti, A. et al. (1999) J. Biol. Chem. 274, 8022-8028. 6 . Lei, M. et al. (2000) Cell 102, 387-397. 7 . Chong, C. et al. (2001) J. Biol. Chem. 276, 17347-17353. 8 . Zhao, Z. et al. (2000) Mol. Cell. Biol. 20, 3906-3917. 9 . Abo, A. et al. (1998) EMBO J. 17, 6527-6540. 10 . Qu, J. et al. (2001) Mol. Cell. Biol. 21, 3523-3533. |
Western blot analysis of extracts from guinea pig neutrophils stimulated with 1 µM fMLP for indicated times, using Phospho-PAK1 (Thr423)/PAK2 (Thr402) Antibody (Provided by Drs. Qian Zhan and John Badwey, Dept. of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Massachusetts.) 使用Phospho-PAK1 (Thr423)/PAK2 (Thr402) Antibody，免疫印迹（Western Blot）分析guinea pig neutrophils细胞中Phospho-PAK1 (Thr423)/PAK2 (Thr402)蛋白水平，组织如图所示给予1 µM fMLP处理。(Provided by Drs. Qian Zhan and John Badwey, Dept. of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Massachusetts.) | |
Western blot analysis of extracts from HeLa cells treated with 0.4 M sorbitol for indicted times, using Phospho-PAK1 (Thr423)/PAK2 (Thr402) Antibody (upper) or PAK2 antibody (lower). 使用Phospho-PAK1 (Thr423)/PAK2 (Thr402) Antibody (上图)或PAK2 antibody (下图)，免疫印迹（Western Blot）分析HeLa细胞中Phospho-PAK1 (Thr423)/PAK2 (Thr402)和PAK2蛋白水平，细胞如图所示时间使用0.4 M sorbitol处理。 |
