| 货号 | 9451T |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit |
| 应用 | WB, IP |
| 目标/特异性 | Phospho-4E-BP1 (Ser65) Antibody detects endogenous levels of 4E-BP1 when phosphorylated at serine 65. This antibody has been shown to also recognize 4E-BP1 when phosphorylated at serine 101 (See application reference, Wang et al. 2003). |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5). |
| 存放说明 | -20C |
| 计算分子量 | 15 to 20 |
| 参考文献 | 1 . Pause, A. et al. (1994) Nature 371, 762-7. 2 . Brunn, G.J. et al. (1997) Science 277, 99-101. 3 . Gingras, A.C. et al. (1998) Genes Dev 12, 502-13. 4 . Fadden, P. et al. (1997) J Biol Chem 272, 10240-7. 5 . Gingras, A.C. et al. (1999) Genes Dev 13, 1422-37. |
Western blot analysis of extracts from 293 cells using 4E-BP1 Antibody #9644 (lower) and Phospho-4E-BP1 (Ser65) Antibody #9451 (upper). The cells were starved for 24 hours in serum-free medium and underwent a 1 hour amino acid deprivation. Amino acids were replenished for 1 hour. Cells were then either untreated (-) or treated with 100 nM insulin (+) for 30 minutes. 使用4E-BP1 Antibody #9644 (下图)和Phospho-4E-BP1 (Ser65) Antibody #9451 (上图)，免疫印迹（Western Blot）分析293细胞裂解物。细胞在无血清饥饿培养24小时，接着氨基酸缺乏培养1小时，然后再加入氨基酸培养1小时。最后细胞加入100 nM胰岛素 (+)或不加(-)处理30分钟。 |
