Phospho-FoxO3a (Ser318/321) Antibody【科瑞奥博】

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Phospho-FoxO3a (Ser318/321) Antibody

货号： 9465T 基本售价： 1600.0 元规格： -

产品信息

概述
货号	9465T
反应种属	Human,Mouse,Rat,Monkey,
来源宿主	Rabbit
应用	WB, IP
目标/特异性	Phospho-Fox03a (Ser318/321) Antibody detects endogenous levels of Fox03a only when phosphorylated at serine 318/321. The antibody is expected to cross-react with Fox01 when phosphorylated at serine 322/325 based on the peptide sequence.
使用方法	WB(1:1000) IP (1:50)

性能
供应商	CST
灵敏度	Endogenous
背景	The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9).Phosphorylation sites Ser322 and Ser325 of Fox01 are important for nuclear export and are homologous to Ser318 and Ser321 of Fox03a (9).
存放说明	-20C
计算分子量	97
参考文献	1 . Anderson, M.J. et al. (1998) Genomics 47, 187-99. 2 . Galili, N. et al. (1993) Nat Genet 5, 230-5. 3 . Borkhardt, A. et al. (1997) Oncogene 14, 195-202. 4 . Nakae, J. et al. (1999) J Biol Chem 274, 15982-5. 5 . Rena, G. et al. (1999) J Biol Chem 274, 17179-83. 6 . Guo, S. et al. (1999) J Biol Chem 274, 17184-92. 7 . Seoane, J. et al. (2004) Cell 117, 211-23. 8 . Arden, K.C. (2004) Mol Cell 14, 416-8. 9 . Yang, Y. et al. (2005) EMBO J 24, 1021-32. 10 . Rena, G. et al. (2002) EMBO J 21, 2263-71.

参考图片

Western blot analysis of extracts from serum starved and serum-treated NIH/3T3 cells as well as untreated and LY294002/Wortmannin-treated MDA-MB-468 cells, using Phospho-Fox03a (Ser318/321) Antibody (upper) or Akt Antibody #9272 (lower).Western blot 分析血清饥饿的或血清处理的NIH/3T3细胞和未处理与LY294002/Wortmannin处理的MDA-MB-468细胞提取物，所用抗体为Phospho-Fox03a (Ser318/321) Antibody (上) or Akt Antibody #9272 (下)。