| 货号 | 2568T |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-LRP6 (Ser1490) Antibody detects endogenous levels of LRP6 protein when phosphorylated at serine 1490. It is expected, although not yet confirmed, to also detect endogenous levels of LRP5 protein when phosphorylated at serine 1493 (corresponding to serine 1490 in LRP6). |
| 使用方法 | WB(1:1000) IP (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | LRP5 and LRP6 are single-pass transmembrane proteins belonging to the low-density lipoprotein receptor (LDLR)-related protein family. Unlike other members of the LDLR family, LRP5 and LRP6 have four EGF and three LDLR repeats in the extracellular domain, and proline-rich motifs in the cytoplasmic domain (1). They function as co-receptors for Wnt and are required for the canonical Wnt/β-catenin signaling pathway (2,3). LRP5 and LRP6 are highly homologous and have redundant roles during development (4,5). The activity of LRP5 and LRP6 can be inhibited by the binding of some members of the Dickkopf (DKK) family of proteins (6,7). Upon stimulation with Wnt, LRP6 is phosphorylated at multiple sites including Thr1479, Ser1490, and Thr1493 by kinases such as GSK-3 and CK1 (8-10). Phosphorylated LRP6 recruits axin to the membrane and presumably activates β-catenin signaling (8-10). |
| 存放说明 | -20C |
| 计算分子量 | 180, 210 |
| 参考文献 | 1 . Brown, S.D. et al. (1998) Biochem. Biophys. Res. Commun. 248, 879-888. 2 . Pinson, K.I. et al. (2000) Nature 407, 535-538. 3 . Tamai, K. et al. (2000) Nature 407, 530-535. 4 . Kelly, O.G. et al. (2004) Development 131, 2803-2815. 5 . He, X. et al. (2004) Development 131, 1663-1677. 6 . Semënov, M.V. et al. (2001) Curr Biol 11, 951-61. 7 . Bafico, A. et al. (2001) Nat. Cell Biol. 3, 683-668. 8 . Tamai, K. et al. (2004) Mol. Cell 13, 149-156. 9 . Zeng, X. et al. (2005) Nature 438, 873-877. 10 . Davidson, G. et al. (2005) Nature 438, 867-872. |
Western blot analysis of total cell lysates from HeLa cells untreated, treated 5 hours with conditioned medium (CM) from L cells, or treated 5 hours with conditioned medium from L cells overexpressing Wnt3a, using Phospho-LRP6 (Ser1490) Antibody #2568. 未处理或经过5小时后条件培养基（CM）处理的L细胞，或在条件培养基处理5小时的过表达Wnt3a的L细胞和HeLa细胞全细胞提取物进行western blot分析，使用Phospho-LRP6 (Ser1490) 抗体#2568。 |
