Cleaved Caspase-9 (Asp315) Antibody (Human Specific)【科瑞奥博】

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Cleaved Caspase-9 (Asp315) Antibody (Human Specific)

货号： 9505T 基本售价： 1600.0 元规格： -

产品信息

概述
货号	9505T
反应种属	Human,
来源宿主	Rabbit
应用	WB, IP
目标/特异性	Cleaved Caspase-9 (Asp315) Antibody (Human Specific) detects endogenous levels of the 35 kDa large fragment of caspase-9 following cleavage at aspartic acid 315. The antibody does not react with full length caspase-9 or any other cleaved caspases.
使用方法	WB(1:1000) IP (1:500)

性能
供应商	CST
灵敏度	Endogenous
背景	Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf 1. Apaf-1 mediated activation of caspase-9 involves instrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Additional cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10).
存放说明	-20C
计算分子量	35
参考文献	1 . Duan, H. et al. (1996) J. Biol. Chem. 271, 16720-16724. 2 . Srinivasula, S. M. et al. (1996) J. Biol. Chem. 271, 27099-27106. 3 . Liu, X. et al. (1996) Cell 86, 147-157. 4 . Li, P. et al. (1997) Cell 91, 479-489. 5 . Zou, H. et al. (1999) J. Biol. Chem. 274, 11549-11556. 6 . Srinivasula, S.M. et al. (1998) Mol Cell 1, 949-57. 7 . Deveraux, Q. L. et al. (1998) EMBO J. 17, 2215-2223. 8 . Slee, E. A. et al. (1999) J. Cell Biol. 144, 281-292. 9 . Sun, X.M. et al. (1999) J Biol Chem 274, 5053-60. 10 . MacFarlane, M. et al. (1997) J. Cell Biol. 137, 469-479.

参考图片

Western blot analysis of extracts from Jurkat cells, untreated or cytochrome c-treated (0.25 mg/ml), and HeLa cells, untreated, staurosporine-treated (1 µM), or cytochrome c-treated (0.25 mg/ml), using Cleaved Caspase-9 (Asp315) Antibody (Human Specific) (upper) or Caspase-9 Antibody (Human Specific) #9502 (lower).

Western blot 分析未处理或cytochrome c（细胞色素C）(0.25 mg/ml)处理的 Jurkat 细胞提取物，以及未处理或staurosporine（星胞菌素） (1 µM)或cytochrome c（细胞色素C）(0.25 mg/ml)处理的HeLa 细胞，抗体采用Cleaved Caspase-9 (Asp315) Antibody (人特异性) (上) 或 Caspase-9 Antibody #9502 (人特异性) (下)。