Cleaved Caspase-9 (Asp353) Antibody (Mouse Specific)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Cleaved Caspase-9 (Asp353) Antibody (Mouse Specific)

货号： 9509T 基本售价： 1600.0 元规格： -

产品信息

概述
货号	9509T
反应种属	Mouse,
来源宿主	Rabbit
应用	WB, IF-IC
目标/特异性	Cleaved Caspase-9 (Asp353) Antibody (Mouse Specific) detects endogenous levels of the 37kDa subunit of mouse caspase-9 only after after cleavage at aspartic acid 353. It does not cross-react with full length caspase-9 or with other caspases at endogenous levels.
使用方法	WB(1:1000) IF-IC (1:50)

性能
供应商	CST
灵敏度	Endogenous
背景	Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf 1. Apaf-1 mediated activation of caspase-9 involves instrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Additional cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10).
存放说明	-20C
计算分子量	37
参考文献	1 . Duan, H. et al. (1996) J. Biol. Chem. 271, 16720-16724. 2 . Srinivasula, S. M. et al. (1996) J. Biol. Chem. 271, 27099-27106. 3 . Liu, X. et al. (1996) Cell 86, 147-157. 4 . Li, P. et al. (1997) Cell 91, 479-489. 5 . Zou, H. et al. (1999) J. Biol. Chem. 274, 11549-11556. 6 . Srinivasula, S.M. et al. (1998) Mol Cell 1, 949-57. 7 . Deveraux, Q. L. et al. (1998) EMBO J. 17, 2215-2223. 8 . Slee, E. A. et al. (1999) J. Cell Biol. 144, 281-292. 9 . Sun, X.M. et al. (1999) J Biol Chem 274, 5053-60. 10 . MacFarlane, M. et al. (1997) J. Cell Biol. 137, 469-479.

参考图片

Confocal immunofluorescent analysis of NIH/3T3 cells, staurosporine-treated (left) or untreated (right), using Cleaved Caspase-9 (Asp353) Antibody (Mouse Specific) (green). Actin filaments have been labeled with Alexa Fluor^® 555 phalloidin (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

共聚焦免疫荧光分析staurosporine（星胞菌素）处理（左）或未处理（右）的NIH/3T3 细胞，抗体采用 Cleaved Caspase-9 (Asp353) Antibody (鼠种特异性) (green)（绿色）。微丝用Alexa Fluor^® 555 phalloidin(鬼比环肽)（红色）标注。蓝色伪彩是DRAQ5^® #4084 （DNA荧光染料）。

Western blot analysis of extracts from L929 cells, untreated or staurosporine-treated, using Cleaved Caspase-9 (Asp353) Antibody (upper) or Caspase-9 Antibody #9504 (lower).

Western blot分析未处理或 staurosporine (星胞菌素)处理的L929 细胞，抗体采用Caspase-9 Antibody (Asp353) (上) 或Caspase-9 Antibody #9504 (下).