| 货号 | 3727T |
| 反应种属 | Human,Mouse,Rat, |
| 来源宿主 | Rabbit |
| 应用 | WB, IP |
| 目标/特异性 | TNF-R2 Antibody detects endogenous levels of total TNF-R2 protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | TNF-α is an important cytokine produced by numerous cell types including neutrophils, activated lymphoctyes, macrophages an NK cells. It plays a critical role in inflammatory responses and in apoptosis (1). TNF-α exists as a membrane-anchored and soluble form, both of which show biological activity. Response to TNF-α is mediated through two receptors, TNF-R1, which is widely expressed, and TNF-R2, which is expressed mainly in immune and endothelial cells (2). Antagonists to TNF-α have been validated as therapeutic targets for rheumatoid arthritis and other immune disorders (3). The two receptors for TNF-α, TNF-R1 (55 kDa) and TNF-R2 (75 kDa) can mediate distinct cellular responses (4,5). In most cases cytotoxicity elicited by TNF has been reported to act throught TNF-R1 (6,7). In contrast, TNF-R2 appears to be important in T cell signaling and responses to infection (7,8). TNF-R2 binds to distinct members of the TRAF family leading to the activation of NF-κB (9,10). Soluble forms of both receptors have also been characterized which can bind TNF-α and may play an important role in immune disorders (11,12). |
| 存放说明 | -20C |
| 计算分子量 | 65 |
| 参考文献 | 1 . Aggarwal, B.B. (2003) Nat Rev Immunol 3, 745-56. 2 . Locksley, R.M. et al. (2001) Cell 104, 487-501. 3 . Taylor, P.C. et al. (2004) Curr Opin Biotechnol 15, 557-63. 4 . Tartaglia, L.A. et al. (1991) Proc Natl Acad Sci U S A 88, 9292-6. 5 . Peschon, J.J. et al. (1998) J Immunol 160, 943-52. 6 . Tartaglia, L.A. et al. (1993) Cell 73, 213-6. 7 . Rothe, J. et al. (1993) Nature 364, 798-802. 8 . Rothe, M. et al. (1995) Science 269, 1424-7. 9 . Aspalter, R.M. et al. (2003) J. Leukoc. Biol. 74, 572-582. 10 . Rothe, M. et al. (1994) Cell 78, 681-92. 11 . Humbert, M. et al. (1994) Am J Respir Crit Care Med 149, 1681-5. 12 . Schröder, J. et al. (1995) Infection 23, 143-8. |
Western blot analysis of extracts from SW620, NK-92 and SR cell lines, using TNF-R2 Antibody. Western blot 检测SW620、NK-92和SR细胞系提取物，使用抗体为TNF-R2 Antibody。 |
