| 货号 | 6882T |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit |
| 应用 | WB, IP , IF-IC |
| 目标/特异性 | RBAP46 (V415) Antibody recognizes endogenous levels of total RBAP46 protein. This antibody does not cross-react with the RBAP48 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:600) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Retinoblastoma-associated proteins 46 and 48 (RBAP46 and RBAP48; also known as RBBP7 and RBBP4) were first characterized in human cells as proteins that bind to the retinoblastoma (Rb) tumor suppressor protein (1). Since then, these proteins have been shown to be components of many protein complexes involved in chromatin regulation, including the chromatin assembly factor 1 (CAF-1) complex and type B histone acetyltransferase complex HAT1, both of which function in chromatin assembly during DNA replication (2,3). RBAP46 and RBAP48 are also found in the nucleosome remodeling factor complex NURF, the nucleosome remodeling and histone de-acetylation complex NuRD, and the Sin3/HDAC histone de-acetylation complex (4-7). More recently, RBAB46 and RBAP48 were identified as components of the polycomb repressor complex PRC2, which also contains EED and Ezh2 (8). RBAP46 and RBAP48 bind to the histone fold region of histone H4 and are believed to target these chromatin remodeling, histone acetylation, and histone de-acetylation complexes to their histone substrates (3). |
| 存放说明 | -20C |
| 计算分子量 | 48 |
| 参考文献 | 1 . Qian, Y.W. et al. (1993) Nature 364, 648-52. 2 . Verreault, A. et al. (1996) Cell 87, 95-104. 3 . Verreault, A. et al. (1998) Curr Biol 8, 96-108. 4 . Barak, O. et al. (2003) EMBO J 22, 6089-100. 5 . Zhang, Y. et al. (1999) Genes Dev 13, 1924-35. 6 . Zhang, Y. et al. (1997) Cell 89, 357-64. 7 . Kuzmichev, A. et al. (2002) Genes Dev 16, 2893-905. 8 . Cao, R. et al. (2002) Science 298, 1039-43. |
Confocal immunofluorescent analysis of HeLa cells using RBAP45 (V415) Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red). 使用RBAP45 (V415) Antibody (绿色)，共聚焦免疫荧光分析HeLa细胞。DY-554 phalloidin标记微丝蛋白(红色)。 | |
Western blot analysis of extracts from various cell lines using RBAP46 (V415) Antibody. 使用RBBP5 Antibody，免疫印迹(Western blot)分析不同细胞中RBAP46 (V415)的蛋白水平。 |
