| 货号 | 2862T |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | c-Abl Antibody detects endogenous levels of Abl proteins. |
| 使用方法 | WB(1:1000) IP (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The c-Abl proto-oncogene encodes a nonreceptor protein tyrosine kinase that is ubiquitously expressed and highly conserved in metazoan evolution. c-Abl protein is distributed in both the nucleus and the cytoplasm of cells. It is implicated in regulating cell proliferation, differentiation, apoptosis, cell adhesion and stress responses (1-3). c-Abl kinase activity is increased in vivo by diverse physiological stimuli including integrin activation, PDGF stimulation and binding to c-Jun, Nck and RFX1 (2,4). The in vivo mechanism of regulation of c-Abl kinase activity is not completely understood. Tyr245 is located in the linker region between the SH2 and catalytic domains. This positioning is conserved among Abl family members. Phosphorylation of Tyr245 is involved in the activation of c-Abl kinase (5). In addition, phosphorylation of Tyr412, which is located in the kinase activation loop of c-Abl, is required for kinase activity (6). |
| 存放说明 | -20C |
| 计算分子量 | 135 (c-Abl); 210 (Bcr-Abl) |
| 参考文献 | 1 . Wang, J.Y. (2000) Oncogene 19, 5643-50. 2 . Van Etten, R.A. (1999) Trends Cell Biol 9, 179-86. 3 . Danial, N.N. and Rothman, P. (2000) Oncogene 19, 2523-31. 4 . Shaul, Y. (2000) Cell Death Differ 7, 10-6. 5 . Brasher, B.B. and Van Etten, R.A. (2000) J Biol Chem 275, 35631-7. 6 . Pluk, H. et al. (2002) Cell 108, 247-259. |
Western blot analysis of extracts from various cell lines, using c-Abl Antibody.western blot方法检测细胞提取物：各种细胞系，使用的抗体是c-Abl Antibody。 |
