| 货号 | 4132T |
| 反应种属 | Human, |
| 来源宿主 | Rabbit |
| 应用 | WB |
| 目标/特异性 | Cyclin E2 Antibody detects endogenous levels of total cyclin E2 protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Cyclin E1 and cyclin E2 can associate with and activate CDK2 (1). Upon DNA damage, upregulation/activation of the CDK inhibitors p21 Waf1/Cip1 and p27 Kip1 prevent cyclin E/CDK2 activation, resulting in G1/S arrest. When conditions are favorable for cell cycle progression, cyclin D/CDK4/6 phosphorylates Rb and is thought to reduce the activity of p21 Waf1/Cip1 and p27 Kip1, allowing subsequent activation of cyclin E/CDK2 (1,2). Cyclin E/CDK2 further phosphorylates Rb to allow progression into S-phase, where cyclin E/CDK2 is thought to phosphorylate and activate multiple proteins involved in DNA synthesis (2,3). Turnover of cyclin E is largely controlled by phosphorylation that results in SCFFbw7-mediated ubiquitination and proteasome-dependent degradation (4,5). Cyclin E1 is phosphorylated at multiple sites in vivo including Thr62, Ser88, Ser72, Thr380 and Ser384, and is controlled by at least two kinases, CDK2 and GSK-3 (6,7).Cyclin E1和Cyclin E2协同并激活CDK2(1)。DNA损伤时，上调/激活CDK抑制基因p21 Waf1/Cip1 and p27 Kip1能够阻滞cyclin E/CDK2的激活，从而导致G1/S期停滞。当条件适宜于细胞周期连续进行时，cyclin D/CDK4/6使Rb磷酸化从而能够降低p21 Waf1/Cip1 和 p27 Kip1的活性，使得cyclin E/CDK2随后激活(1,2)。Cyclin E/CDK2进一步磷酸化Rb使细胞周期进入S期，在此期内cyclin E/CDK2被认为可以磷酸化并激活涉及DNA合成过程的多种蛋白(2,3)。周期蛋白E的功能转化主要受磷酸化控制，这种磷酸化能够导致SCFFbw7介导的泛素化和蛋白酶体依赖的降解(4,5)。Cyclin E1在体内可以在包括苏氨酸62位，丝氨酸88位，丝氨酸72位，苏氨酸380位和丝氨酸384位的多位点磷酸化，这个磷酸化过程至少可以被两个激酶-CDK2和GSK-3调控 |
| 存放说明 | -20C |
| 计算分子量 | 48 |
| 参考文献 | 1 . Lauper, N. et al. (1998) Oncogene 17, 2637-43. 2 . Lundberg, A.S. and Weinberg, R.A. (1998) Mol Cell Biol 18, 753-61. 3 . Ewen, M.E. (2000) Genes Dev 14, 2265-70. 4 . Won, K.A. and Reed, S.I. (1996) EMBO J 15, 4182-93. 5 . Koepp, D.M. et al. (2001) Science 294, 173-7. 6 . Welcker, M. et al. (2003) Mol Cell 12, 381-92. 7 . Ye, X. et al. (2004) J Biol Chem 279, 50110-9. |
Western blot analysis of extracts from MCF-7, SK-N-MC and HeLa cells, untreated or treated with the proteasome inhibitor MG-132, using Cyclin E2 Antibody.western blot方法检测未使用和使用蛋白酶体抑制剂MG-132处理的MCF-7, SK-N-MC 和 HeLa 细胞提取物，使用的抗体为Cyclin E2 Antibody。 |
