| 货号 | 3087T |
| 反应种属 | Human,Mouse, |
| 来源宿主 | Rabbit |
| 应用 | WB |
| 目标/特异性 | C/EBPbeta (LAP) Antibody detects endogenous levels of total C/EBPbeta, the p38 and p36 LAPs, but not the p20 LIP. This antibody does not cross-react with C/EBPalpha, -delta, -gamma, -epsilon or -zeta. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | CCAAT/enhancer-binding proteins (C/EBPs) are a family of transcription factors critical for cellular differentiation, terminal functions and inflammatory response (1). Six members of the family have been characterized (C/EBPα, -β, -γ, -δ, -ε and -ζ) and are distributed in a variety of tissues (1). There are two forms of C/EBPβ, the 38 kDa liver activating protein (LAP) and the 20 kDa liver inhibitory protein (LIP) which may be products of alternative translation. The 38 kDa LAP protein is a transcriptional activator while LIP may act as an inhibitor of C/EBPβ transcriptional activity (2). Phosphorylation of C/EBPβ at distinct sites stimulates its transcriptional activity (3-5). Phosphorylation at serine 105 of rat C/EBPβ, a unique site only present in the rat sequence, seems essential for rat C/EBPβ activation (6). |
| 存放说明 | -20C |
| 计算分子量 | 35 to 38 mouse LAP. 45 to 49 human LAP. |
| 参考文献 | 1 . Lekstrom-Hims, J. and Xanthopoulos, K.G. (1998) J. Biol. Chem. 273, 28545-28548. 2 . Calkhoven, C.F. et al. (2000) Genes Dev 14, 1920-32. 3 . Wegner, M. et al. (1992) Science 256, 370-3. 4 . Trautwein, C. et al. (1993) Nature 364, 544-7. 5 . Nakajima, T. et al. (1993) Proc Natl Acad Sci U S A 90, 2207-11. 6 . Buck, M. et al. (1999) Mol Cell 4, 1087-92. |
Western blot analysis of extracts from COS cells, untransfected or transfected with human or mouse C/EBPbeta (LAP), using C/EBPbeta (LAP) Antibody. 对COS细胞，未转染或转染人或小鼠C/EBPbeta (LAP)，使用C/EBPbeta (LAP)抗体进行Western blot分析。 | |
Western blot analysis of extracts from NIH/3T3-L1, differentiated for the indicated times, using C/EBPbeta (LAP) Antibody. 对NIH/3T3-L1细胞（分化适当时间）抽提液，使用C/EBPbeta (LAP)抗体进行Western blot分析。 |
