| 货号 | 3142T |
| 反应种属 | Human/Mouse/Rat/Monkey/Bovine |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Ezrin/Radixin/Moesin Antibody detects endogenous levels of ezrin, radixin and moesin. The antibody does not cross-react with related proteins such as merlin or band 4.1. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The ezrin, radixin, and moesin (ERM) proteins function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling, and microvilli formation (1). ERM proteins undergo intra or intermolecular interaction between their amino- and carboxy-terminal domains, existing as inactive cytosolic monomers or dimers (2). Phosphorylation at a carboxy-terminal threonine residue (Thr567 of ezrin, Thr564 of radixin, Thr558 of moesin) disrupts the amino- and carboxy-terminal association and may play a key role in regulating ERM protein conformation and function (3,4). Phosphorylation at Thr567 of ezrin is required for cytoskeletal rearrangements and oncogene-induced transformation (5). Ezrin is also phosphorylated at tyrosine residues upon growth factor stimulation. Phosphorylation of Tyr353 of ezrin transmits a survival signal during epithelial differentiation (6). |
| 存放说明 | -20C |
| 计算分子量 | 75 Moesin. 80 Ezrin and Radixin. |
| 参考文献 | 1 . Tsukita, S. and Yonemura, S. (1999) J Biol Chem 274, 34507-10. 2 . Mangeat, P. et al. (1999) Trends Cell Biol 9, 187-92. 3 . Matsui, T. et al. (1998) J Cell Biol 140, 647-57. 4 . Gautreau, A. et al. (2000) J Cell Biol 150, 193-203. 5 . Tran Quang, C. et al. (2000) EMBO J 19, 4565-76. 6 . Gautreau, A. et al. (1999) Proc Natl Acad Sci U S A 96, 7300-5. |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Ezrin/Radixin/Moesin Antibody.使用Ezrin/Radixin/Moesin Antibody，免疫组化分析人源结肠癌组织石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Ezrin/Radixin/Moesin Antibody in the presence of control peptide (left) or antigen-specific peptide (right).使用Ezrin/Radixin/Moesin Antibody，免疫组化分析人源乳腺癌组织石蜡切片，其分别被孵育control peptide (左图)或antigen-specific peptide (右图)。 | |
Western blot analysis of extracts from various cell lines using Ezrin/Radixin/Moesin Antibody.使用Ezrin/Radixin/Moesin Antibody，免疫印迹（Western Blot）分析不同细胞中Ezrin/Radixin/Moesin蛋白水平。 |
