| 货号 | 3218T |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey/Mink/Bovine |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Dvl3 Antibody detects endogenous levels of total Dvl3 protein. This antibody does not cross-react with Dvl2. |
| 使用方法 | WB(1:1000) IP (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Dishevelled (Dsh) proteins are important intermediates of Wnt signaling pathways. Dsh inhibits glycogen synthase kinase-3β promoting β-catenin stabilization. Dsh proteins also participate in the planar cell polarity pathway by acting through JNK (1,2). There are three Dsh homologs, Dvl1, Dvl2 and Dvl3 in mammals. Upon treatment with Wnt proteins, Dvls become hyperphosphorylated (3) and accumulate in the nucleus (4). Dvl proteins also associate with actin fibers and cytoplasmic vesicular membranes (5) and mediate endocytosis of the Fzd receptor after Wnt protein stimulation (6). Overexpression of Dvl has been reported in certain cancers (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 88 to 93 |
| 参考文献 | 1 . Logan, C.Y. and Nusse, R. (2004) Annu Rev Cell Dev Biol 20, 781-810. 2 . Boutros, M. and Mlodzik, M. (1999) Mech. Dev. 83, 27-37. 3 . Lee, J.S. et al. (1999) J. Biol. Chem. 274, 21464-70. 4 . Itoh, K. et al. (2005) J. Biol. 4, 3.1-3.12. 5 . Capelluto, D.G. et al. (2002) Nature 419, 726-729. 6 . Chen, W. et al. (2003) Science 301, 1391-1394. 7 . Okino , K. et al. (2003) Oncol. Rep. 10, 1219-1223. 8 . Uematsu, K. et al. (2003) Oncogene 22, 7218-7221. |
Western blot analysis of extracts from NIH/3T3, C6, A549, BAEC and COS cells using Dvl3 Antibody.使用Dvl3抗体对NIH/3T3, C6, A549, BAEC和 COS 细胞提取物进行western blot分析。 |
