| 货号 | 3236T |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | α-E-Catenin Antibody detects endogenous levels of alpha-E catenin protein. The antibody may cross-react with neuronal α-N-catenin. In some cell lysates, the antibody may cross-react with a 50 kDa band of unknown origin. |
| 使用方法 | WB(1:1000) IF-IC (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Adherens junctions are dynamic structures that form cell-cell contacts and are important in development, differentiation, tissue integrity, morphology and cell polarity. They are composed of the transmembrane proteins, cadherins, which bind cadherins on adjacent cells in a calcium-dependent manner. On the cytoplasmic side of adherens junctions, the classic model states that cadherins are linked to the cytoskeleton through β- and α-catenin. α-E-catenin is ubiquitously expressed, α-N-catenin is expressed in neuronal tissue, and α-T-catenin is primarily expressed in heart tissue. Loss of E-cadherin and α-E-catenin occurs during the progression of several human cancers, indicating that the breakdown of adherens junctions is important in cancer progression (reviewed in 1). Recent evidence suggests that, rather than acting as a static link between cadherins and actin, α-catenin regulates actin dynamics directly, possibly by competing with the actin nucleating arp2/3 complex (2,3). α-catenin also plays a role in regulating β-catenin-dependent transcriptional activity, affecting differentiation and response to Wnt signaling. α-catenin binds to β-catenin in the nucleus, preventing it from regulating transcription, and levels of both proteins appear to be regulated via proteasome-dependent degradation (4). |
| 存放说明 | -20C |
| 计算分子量 | 100 |
| 参考文献 | 1 . Kobielak, A. and Fuchs, E. (2004) Nat Rev Mol Cell Biol 5, 614-25. 2 . Yamada, S. et al. (2005) Cell 123, 889-901. 3 . Drees, F. et al. (2005) Cell 123, 903-15. 4 . Hwang, S.G. et al. (2005) J Biol Chem 280, 12758-65. |
Confocal immunofluorescent analysis of A-431 cells labeled with α-E-Catenin Antibody (green, left) compared to an isotype control (right). Actin filaments have been labeled with phalloidin (red). Blue pseudocolor = TO-PRO-3 (fluorescent DNA dye).使用α-E-Catenin 抗体（绿色，左）对比同型对照（右）对A-431细胞进行激光共聚焦免疫荧光分析。肌动蛋白丝使用鬼笔环肽（红色）标记。蓝色假色=TO-PRO-3（DNA荧光染料）。 | |
Western blot analysis of extracts from various cell lines, using α-E-Catenin Antibody.使用α-E-Catenin 抗体对多种细胞提取物进行western blot分析。 |
