| 货号 | 4976T |
| 反应种属 | Human, |
| 来源宿主 | Rabbit |
| 应用 | WB |
| 目标/特异性 | Puma Antibody detects endogenous levels of total Puma protein. The antibody also cross-reacts with an 18kDa band of unknown origin. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Puma (p53 upregulated modulator of apoptosis) is a "BH3-only" Bcl-2 family member originally identified in differential gene expression studies as a p53-inducible gene (1,2). The "BH3-only" family members include Bad, Bid, Bik, Hrk, Bim and Noxa, all of which contain a BH3 domain but lack other conserved domains, BH1 and BH2, and generally promote apoptosis by binding to and antagonizing anti-apoptotic Bcl-2 family members through BH3 domain interactions (3). Two BH3-containing proteins are produced from the puma gene, Puma-α and Puma-β, both of which are induced by p53, bind Bcl-2 and Bcl-xL, localize to the mitochondria and promote cyctochrome c release and apoptosis (1,2). Puma plays a critical role in the p53 tumor suppressor pathway. Targeted disruption of the puma gene impairs p53-mediated apoptosis and tumor suprression (4-7). Puma knockout mice show defects from multiple apoptotic stimuli, including ionizing irradiation, deregulated c-Myc expression, and cytokine withdrawal (4). |
| 存放说明 | -20C |
| 计算分子量 | 23 |
| 参考文献 | 1 . Yu, J. et al. (2001) Mol Cell 7, 673-82. 2 . Nakano, K. and Vousden, K.H. (2001) Mol Cell 7, 683-94. 3 . Bouillet, P. and Strasser, A. (2002) J Cell Sci 115, 1567-74. 4 . Jeffers, J.R. et al. (2003) Cancer Cell 4, 321-8. 5 . Hemann, M.T. et al. (2004) Proc Natl Acad Sci U S A 101, 9333-8. 6 . Yu, J. et al. (2003) Proc Natl Acad Sci U S A 100, 1931-6. 7 . Villunger, A. et al. (2003) Science 302, 1036-8. |
Western blot analysis of extracts from various cells using Puma Antibody. Western blot 检测多种细胞系提取物，使用抗体为Puma Antibody。 |
