| 货号 | 8559T |
| 反应种属 | Human,Monkey, |
| 来源宿主 | Rabbit |
| 应用 | WB |
| 目标/特异性 | TPX2 Antibody recognizes endogenous levels of total TPX2 protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The Ras family small GTPase Ran is involved in nuclear envelope formation, assembly of the mitotic spindle, and nuclear transport (1,2). TPX2, a target of active Ran (RanGTP), is a microtubule nucleating protein. TPX2 is inactive when bound to nuclear importin-alpha. RanGTP activity disrupts this interaction, relieving inhibition of TPX2 (3). TPX2 binding activates Aurora A kinase and promotes its localization to the mitotic spindle (4,5). DNA damage in mitosis leads to loss of interaction between Aurora A and TPX2 and inactivation of Aurora A kinase (6). TPX2 is highly expressed in pancreatic cancer cells, and knockdown of TPX2 expression in these cells is associated with increased sensitivity to paclitaxel (7).Ras家族小GTP酶Ran参与核被膜形成、有丝分裂纺锤体组装和核运输(1,2)。TPX2，活化Ran(RanGTP)的一个靶标，是一种微管核化蛋白。当结合到核输入因子α时TPX2是失活的。RanGTP的活性破坏了这种相互作用，解除了对TPX2的抑制(3)。TPX2结合激活Aurora A激酶并促进其向有丝分裂纺锤体定位(4,5)。有丝分裂过程中的DNA 损伤导致了Aurora A和TPX2相互作用的缺失以及Aurora A激酶的失活(6)。TPX2在胰腺癌细胞中高表达，敲除TPX2后这些细胞对紫杉醇的敏感性增加(7)。 |
| 存放说明 | -20C |
| 计算分子量 | 100 |
| 参考文献 | 1 . Quimby, B.B. and Dasso, M. (2003) Curr Opin Cell Biol 15, 338-44. 2 . Hetzer, M. et al. (2002) Nat Cell Biol 4, E177-84. 3 . Gruss, O.J. and Vernos, I. (2004) J Cell Biol 166, 949-55. 4 . Kufer, T.A. et al. (2002) J Cell Biol 158, 617-23. 5 . Bayliss, R. et al. (2004) Cell Cycle 3, 404-7. 6 . Bhatia, P. et al. (2010) Cell Cycle 9, 4592-9. 7 . Warner, S.L. et al. (2009) Clin Cancer Res 15, 6519-28. |
Western blot analysis of extracts from various cell lines using TPX2 Antibody.Western blot方法检测不同细胞提取物，使用的抗体是TPX2 Antibody。 |
