| 货号 | 3363T |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Dicer Antibody detects endogenous levels of total Dicer protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Dicer is a member of the RNase III family that specifically cleaves double-stranded RNAs to generate microRNAs (miRNAs) (1). After long primary transcript pri-miRNAs are processed to stem-looped pre-miRNAs by Drosha (2), pre-miRNAs are transported to the cytoplasm and further processed by Dicer to produce 22-nucleotide mature miRNAs (3). The mature miRNA then becomes a part of the RNA-Induced Silencing Complex (RISC) and can bind to the 3 UTR of the target mRNA (3). |
| 存放说明 | -20C |
| 计算分子量 | 220 |
| 参考文献 | 1 . Hutvágner, G. and Zamore, P.D. (2002) Science 297, 2056-60. 2 . Lee, Y. et al. (2003) Nature 425, 415-9. 3 . Diederichs, S. and Haber, D.A. (2007) Cell 131, 1097-108. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® Dicer siRNA I #6576 (+) or SignalSilence® Dicer siRNA II (+), using Dicer Antibody #3363 and α-Tubulin (11H10) Rabbit mAb #2125. The dicer antibody confirms silencing of dicer expression, while α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of dicer siRNA. 使用Dicer Antibody #3363和α-Tubulin (11H10) Rabbit mAb兔单抗 #2125，免疫印迹（Western Blotting）分析HeLa细胞蛋白提取物，其分别转染100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® Dicer siRNA I #6576 (+)和SignalSilence® Dicer siRNA II (+)。使用α-Tubulin (11H10) Rabbit mAb兔单抗作为上样和Dicer siRNA特异性的参照，Dicer抗体确证Dicer基因表达沉默。 | |
Western blot analysis of extracts from various cell types using Dicer Antibody. 使用Dicer Antibody，免疫印迹（Western Blotting）分析不同细胞系提取物。 |
