| 货号 | 3403T |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | Myosin IIa Antibody detects endogenous levels of total myosin IIa protein. The antibody does not cross-react with the nonmuscle heavy chains of myosin IIb or IIc. |
| 使用方法 | WB(1:1000) IF-IC (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Nonmuscle myosin is an actin-based motor protein essential to cell motility, cell division, migration, adhesion, and polarity. The holoenzyme consists of two identical heavy chains and two sets of light chains. The light chains (MLCs) regulate myosin II activity and stability. The heavy chains (NMHCs) are encoded by three genes, MYH9, MYH10, and MYH14, which generate three different nonmuscle myosin II isoforms, IIa, IIb, and IIc, respectively (reviewed in 1). While all three isoforms perform the same enzymatic tasks, binding to and contracting actin filaments coupled to ATP hydrolysis, their cellular functions do not appear to be redundant and they have different subcellular distributions (2-5). The carboxy-terminal tail domain of myosin II is important in isoform-specific subcellular localization (6). Phosphorylation of myosin IIa at Ser1943 contributes to the regulation of breast cancer cell migration (7). |
| 存放说明 | -20C |
| 计算分子量 | 230 |
| 参考文献 | 1 . Conti, M.A. and Adelstein, R.S. (2008) J Cell Sci 121, 11-18. 2 . Sandquist, J.C. et al. (2006) J Biol Chem 281, 35873-83. 3 . Even-Ram, S. et al. (2007) Nat Cell Biol 9, 299-309. 4 . Vicente-Manzanares, M. et al. (2007) J Cell Biol 176, 573-80. 5 . Wylie, S.R. and Chantler, P.D. (2008) Mol Biol Cell 19, 3956-68. 6 . Sandquist, J.C. and Means, A.R. (2008) Mol Biol Cell 19, 5156-67. 7 . Dulyaninova, N.G. et al. (2007) Mol Biol Cell 18, 3144-55. |
Confocal immunofluorescent analysis of HeLa cells using Myosin IIa Antibody (green) showing colocalization with actin filaments that have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). 使用Myosin IIa Antibody (绿色)标记进行共聚焦免疫荧光分析HeLa细胞，结果显示与肌动蛋白微丝共定位，而DY-554 phalloidin标记微丝蛋白（红色）。蓝色伪彩= DRAQ5™（DNA荧光染料）。 | |
Western blot analysis of extracts from various cell types using Myosin IIa Antibody. 使用Myosin IIa Antibody，免疫印迹（Western Blot）分析不同细胞中Myosin IIa蛋白水平。 | |
Western blot analysis of extracts from COS cells, mock transfected or transfected with nonmuscle EGFP-myosin IIa, IIb or IIc, using Myosin IIa Antibody. 使用Myosin IIa Antibody，免疫印迹（Western Blot）分析COS细胞中Myosin IIa蛋白水平。细胞分为mock transfected、转染EGFP-myosin IIa、IIb或IIc。 |
