| 货号 | 3508T |
| 反应种属 | Human,Mouse,Monkey, |
| 来源宿主 | Rabbit |
| 应用 | WB, IF-IC |
| 目标/特异性 | Brg1 (A52) Antibody detects endogenous levels of Brg1 protein. |
| 使用方法 | WB(1:1000) IF-IC (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The modulation of chromatin structure is an essential component in the regulation of transcriptional activation and repression. Modifications can be made by at least two evolutionarily conserved strategies, through the disruption of histone-DNA contacts by ATP-dependent chromatin remodelers, or by histone tail modifications including methylation and acetylation. One of the four classes of ATP-dependent histone remodelers is the SWI/SNF complex, the central catalytic subunit of which is Brg1 or the highly related protein hBRM (1). This SWI/SNF complex contains varying subunits but its association with either Brg1 or hBRM remains constant (1). SWI/SNF complexes have been shown to regulate gene activation, cell growth, the cell cycle and differentiation (1). Brg1/hBRM have been shown to regulate transcription through enhancing transcriptional activation of glucocorticoid receptors (2). Although usually associated with transcriptional activation, Brg1/hBRM have also been found in complexes associated with transcriptional repression including with HDACs, Rb and Tif1β (3-5). Brg1/hBRM plays a vital role in the regulation of gene transcription during early mammalian embryogenesis. In addition, Brg1/hBRM also play a role as a tumor suppressors and Brg1 is mutated in several tumor cell lines (6-8). |
| 存放说明 | -20C |
| 计算分子量 | 220 |
| 参考文献 | 1 . Trotter, K.W. and Archer, T.K. (2008) Nucl Recept Signal 6, e004. 2 . Trotter, K.W. and Archer, T.K. (2007) Mol Cell Endocrinol 265-266, 162-7. 3 . Sif, S. et al. (2001) Genes Dev 15, 603-18. 4 . Zhang, H.S. et al. (2000) Cell 101, 79-89. 5 . Underhill, C. et al. (2000) J Biol Chem 275, 40463-70. 6 . Magnani, L. and Cabot, R.A. (2009) Reproduction 137, 23-33. 7 . Medina, P.P. et al. Epigenetics 3, 64-8. 8 . Medina, P.P. et al. (2008) Hum Mutat 29, 617-22. |
Confocal immunofluorescent analysis of 293 cells using Brg1 (A52) Antibody (green). Actin filaments have been labeled using DY-554 phalloidin (red). 使用Brg1 (A52) Antibody (绿色)，共聚焦免疫荧光分析293细胞。DY-554 phalloidin标记微丝蛋白(红色)。 | |
Western blot analysis of extracts of various cell lines using Brg1 (A52) Antibody. 使用Brg1 (A52) Antibody，免疫印迹(Western blot)分析不同细胞中Brg1 (A52)的蛋白水平。 |
