XIAP Antibody【科瑞奥博】

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XIAP Antibody

货号： 2042T 基本售价： 1350.0 元规格： -

产品信息

概述
货号	2042T
反应种属	Human,Mouse,Rat,Monkey,
来源宿主	Rabbit
应用	WB
目标/特异性	XIAP Antibody detects endogenous levels of total XIAP protein. The antibody does not cross-react with other inhibitors of apoptosis.
使用方法	WB(1:1000)

性能
供应商	CST
灵敏度	Endogenous
背景	The inhibitor of apoptosis protein (IAP) family consists of an evolutionarily conserved group of apoptosis inhibitors containing a conserved 70 amino acid BIR (baculovirus inhibitor repeat) domain (1,2). Human members of this family include c-IAP1, c-IAP2, XIAP, survivin, livin, and NAIP. Overexpression of IAP family members, particularly survivin and livin, in cancer cell lines and primary tumors suggests an important role for these proteins in cancer progression (3-5). In general, the IAP proteins function through direct interactions to inhibit the activity of several caspases, including caspase-3, caspase-7, and caspase-9 (5,6). In addition, binding of IAP family members to the mitochondrial protein Smac blocks their interaction with caspase-9, thereby allowing the processing and activation of the caspase (2).
存放说明	-20C
计算分子量	53
参考文献	1 . Deveraux, Q.L. and Reed, J.C. (1999) Genes Dev 13, 239-52. 2 . Deveraux, Q. L. et al. (1998) EMBO J. 17, 2215-2223. 3 . Altieri, D.C. et al. (1999) Lab Invest 79, 1327-33. 4 . Tamm, I. et al. (2000) Clin Cancer Res 6, 1796-803. 5 . Kasof, G.M. and Gomes, B.C. (2001) J Biol Chem 276, 3238-46. 6 . Deveraux, Q.L. et al. (1997) Nature 388, 300-4.

参考图片

Western blot analysis of extracts from HeLa (human), C6 (rat) and BaF3 (mouse) cell lines, using XIAP Antibody.

Western blot检测HeLa（人）、C6 （大鼠）和BaF3（小鼠）细胞系提取物，使用抗体为XIAP Antibody。

Western blot analysis of extracts from A549 cells 48 hours following mock transfection (-), transfection with non-targeted (control) siRNA or transfection with XIAP siRNA. XIAP was detected using XIAP Antibody #2042, and cofilin was detected using Cofilin Antibody #3312. The XIAP Antibody confirms silencing of XIAP expression, and the cofilin Antibody is used to control for protein loading and siRNA specificity.

Western blot方法检测A549细胞提取物，用空质粒(-)、非靶向 (对照) siRNA或100 nM XIAP siRNA转染48hr后提取蛋白；使用XIAP Antibody #2042 (上图) 检测XIAP，使用Cofilin Antibody #3312 (下图)检测cofilin。XIAP Antibody确定XIAP表达的沉默，cofilin Antibody作为内参对照。

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence^® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence^® XIAP siRNA II (+), using XIAP Antibody #2042 and β-Actin Antibody #4967. The XIAP antibody confirms silencing of XIAP expression, while the β-Actin Antibody is used to control for loading and specificity of XIAP siRNA.

Western blot方法检测HeLa细胞提取物，100 nM SignalSilence^® Control siRNA (Fluorescein Conjugate) #6201 (-)或SignalSilence^® XIAP siRNA II (+)转染；使用XIAP Antibody #2042或β-Actin Antibody #4967。XIAP antibody确定XIAP表达的沉默，β-Actin Antibody作为XIAP siRNA的内参对照。