| 货号 | 4107T |
| 反应种属 | Mouse, |
| 来源宿主 | Rabbit |
| 应用 | WB, IP , IF-IC |
| 目标/特异性 | HSL Antibody detects endogenous levels of total HSL protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | HSL (hormone-sensitive lipase) catalyzes the hydrolysis of triacylglycerol, the rate-limiting step in lipolysis. Lipolytic stimuli activate adenylyl cyclase and thus increase intracellular cAMP levels, which in turn activate protein kinase A (PKA). PKA phosphorylates HSL at Ser563, Ser659 and Ser660, which stimulates HSL activity (1,2). In contrast, AMPK phosphorylates HSL at Ser565, which reduces HSL phosphorylation at Ser563 by PKA and inhibits HSL activity (2,3). Recent work indicates that phosphorylation at Ser600 by p44/42 MAPKs also enhances the enzymatic activity of HSL (4). |
| 存放说明 | -20C |
| 计算分子量 | 81, 83 |
| 参考文献 | 1 . Degerman, E. et al. (1990) Proc. Natl. Acad. Sci. USA 87, 533-37. 2 . Anthonsen, M.W. et al. (1998) J. Biol. Chem. 273, 215-21. 3 . Garton, A.J. and Yeaman, S.J. (1990) Eur. J. Biochem. 191, 245-50. 4 . Greenberg, A.S. et al. (2001) J. Biol. Chem. 276, 45456-61. |
Western blot analysis of extracts from NIH/3T3 and 3T3-L1 cells using HSL Antibody. Wetern blot检测NIH/3T3和3T3-L1 细胞提取物，所用抗体为HSL Antibody。 |
