| 货号 | 4282T |
| 反应种属 | Human,Mouse,Rat, |
| 来源宿主 | Rabbit |
| 应用 | WB, IHC-P |
| 目标/特异性 | Myt1 Antibody detects endogenous levels of total Myt1 protein. |
| 使用方法 | WB(1:1000) IHC-P (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. The critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Thr14 (1,2). Phosphorylation at Tyr15 and Thr14 and inhibition of cdc2 is carried out by Wee1 and Myt1 protein kinases, while Tyr15 dephosphorylation and activation of cdc2 is carried out by the cdc25 phosphatase (1,3,4). Hyperphosphorylation and inactivation of Myt1 in mitosis suggests that one or more kinases activated at the G2/M transition negatively regulates Myt1 activity. Kinases shown to phosphorylate Myt1 include cdc2, p90RSK, Akt, and Plk1 (5-8).cdc2激酶的激活参与调节所有的真核细胞进入有丝分裂。进入有丝分裂过程中cdc2激活的关键调节步骤似乎是酪氨酸15位和14位的去磷酸化(1,2)。Wee1和Myt1蛋白激酶可以磷酸化酪氨酸15位和14位并抑制cdc2，而cdc25磷酸酶能够去磷酸化酪氨酸15位并激活cdc2(1,3,4)。有丝分裂中Myt1的过磷酸化和失活表明一个或多个激酶在G2/M转换期激活并负调控Myt1的活性。能够磷酸化Myt1的激酶有cdc2, p90RSK, Akt和Plk1 (5-8)。 |
| 存放说明 | -20C |
| 计算分子量 | 60 to 70 |
| 参考文献 | 1 . Watanabe, N. et al. (1995) EMBO J. 14, 1878-1891. 2 . Hunter, T. (1995) Cell 80, 225-36. 3 . Galaktionov, K. et al. (1995) Genes Dev 9, 1046-58. 4 . McGowan, C.H. and Russell, P. (1993) EMBO J 12, 75-85. 5 . Booher, R.N. et al. (1997) J Biol Chem 272, 22300-6. 6 . Palmer, A. et al. (1998) EMBO J 17, 5037-47. 7 . Palmer, A. et al. (1998) EMBO J 17, 5037-47. 8 . Nakajima, H. et al. (2003) J Biol Chem 278, 25277-80. |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Myt1 Antibody.免疫组织化学方法检测石蜡包埋人类肺癌组织，使用的抗体为Myt1 Antibody。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Myt1 Antibody in the presence of control peptide (left) or antigen specific peptide (right).免疫组织化学方法检测石蜡包埋人类结肠癌组织，左图为对照多肽，右图为抗原特异性多肽，使用的抗体为Myt1 Antibody。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Myt1 Antibody.免疫组织化学方法检测石蜡包埋人类结肠癌组织，使用的抗体为Myt1 Antibody。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear and cytoplasmic localization using Myt1 Antibody.免疫组织化学方法检测石蜡包埋人类乳腺癌组织，显示核定位和胞浆定位，使用的抗体为Myt1 Antibody。 | |
Western blot analysis of extracts from HT29 cells, untreated (lane 1), nocodazole-treated (50 ng/ml, lane 2), or UV-treated (lane 3), using Myt1 Antibody.western blot方法检测HT29细胞提取物：未处理组（泳道1），nocodazole处理组(50 ng/ml, 泳道 2), 紫外处理组（泳道3），使用的抗体为Myt1 Antibody。 |
