| 货号 | 4363T |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit |
| 应用 | WB, IP |
| 目标/特异性 | IRAK4 Antibody detects endogenous levels of total IRAK4. Cross-reactivity was not detected with other family members. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Interleukin-1 (IL-1) receptor-associated kinase (IRAK) is a serine/threonine-specific kinase that can be coprecipitated in an IL-1-inducible manner with the IL-1 receptor (1). The mammalian family of IRAK molecules contains four members (IRAK1, IRAK2, IRAK3/IRAK-M and IRAK4). The binding of IL-1 to IL-1 receptor type I (IL-1RI) initiates the formation of a complex that includes IL-1RI, AcP, MyD88 and IRAKs (2). IRAK undergoes autophosphorylation shortly after IL-1 stimulation. The subsequent events involve IRAK dissociation from the IL-1RI complex, its ubiquitination and its association with two membrane-bound proteins: TAB2 and TRAF6. The resulting IRAK-TRAF6-TAB2 complex is then released into the cytoplasm and activates protein kinase cascades, which include TAK1, IKKs and the stress-activated kinases (3). |
| 存放说明 | -20C |
| 计算分子量 | 55 |
| 参考文献 | 1 . Dinarello, C.A. (1996) Blood 87, 2095-147. 2 . Takaesu, G. et al. (2001) Mol Cell Biol 21, 2475-84. 3 . Janssens, S. and Beyaert, R. (2003) Mol Cell 11, 293-302. 4 . Gottipati, S. et al. (2008) Cell Signal 20, 269-76. 5 . Kollewe, C. et al. (2004) J Biol Chem 279, 5227-36. |
Western blot analysis of extracts from THP-1 (human), RAW 264.7 (mouse), and H-4-II-E (rat) cell lines, using IRAK4 Antibody.Western免疫印迹。用IRAK4 Antibody研究THP-1(人源), RAW 264.7 (小鼠源)和 H-4-II-E (大鼠源)细胞系的细胞提取液。 |
