| 货号 | 4502T |
| 反应种属 | Human,Mouse,Rat, |
| 来源宿主 | Rabbit |
| 应用 | WB |
| 目标/特异性 | IRS-2 Antibody detects endogenous levels of total IRS-2 protein. It does not cross-react with IRS-1. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Insulin Receptor Substrate 2 (IRS-2) is one of the major substrates of the insulin receptor kinase (1). In vertebrates, IRS-2 functions as a scaffolding protein to coordinate separate branches of the Insulin/IGF-signaling cascades (2). IRS-2 is essential for normal nutrient homeostasis because it mediates both peripheral insulin action and the effect of IGF-1 on B-cell growth. Mice lacking IRS-2 fail to maintain sufficient compensatory insulin secretion and develop diabetes as young adults (3). |
| 存放说明 | -20C |
| 计算分子量 | 185 |
| 参考文献 | 1 . Sun, X.J. et al. (1991) Nature 352, 73-77. 2 . White, M.F. (2002) Am. J. Physiol. Endocrinol. Metab. 283, E413-E422. 3 . Withers, D.J. et al. (1998) Nature 391, 900-904. |
Western blot analysis of extracts from CHO IR/4PS cells (transfected with insulin receptor and IRS-2) or CHO IR/IRS-1 cells (transfected with insulin receptor and IRS-1), showing no change in total protein levels with insulin stimulation (100 nM for 5 min), using IRS-2 Antibody (top) or IRS-1 Antibody #2382 (bottom). Wetern blot检测CHO IR/4PS 和CHO IR/IRS1提取物，显示经胰岛素处理后总蛋白水平没有变化，所用抗体为IRS-2 Antibody (上)和 IRS-1 Antibody #2382(下)。 |
