| 货号 | 4294T |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit |
| 应用 | WB |
| 目标/特异性 | PHLDA3 Antibody detects endogenous level of total PHLDA3 protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Pleckstrin homology-like domain family A member 3 (PHLDA3) is one of three relatively small, similar proteins that share a common pleckstrin homology (PH) domain. Expression of PHLDA3 and the related PHLDA1 occurs in several fetal and adult tissues, in contrast to the more restricted expression of PHLDA2 seen in mouse tissues (1). PHLDA3 is one of several proteins induced following treatment of tumor cells with cisplatin, an anti-cancer drug that cross-links DNA and promotes apoptosis through activation of the tumor suppressor p53 (2). Additional evidence that PHLDA3 is involved in promoting apoptosis through p53 came from a study examining the opposing effects of p53 and Akt in tumor development. Tumor suppressor p53 binds the PHLDA3 promoter to induce transcription. Induced overexpression of PHLDA3 increases apoptosis while deletion of PHLDA3 results in increased Akt activity and a reduction in p53-mediated apoptosis. PHLDA3 appears to compete with the PH domain of Akt, preventing Akt activation and promotion of Akt-induced cell survival pathways (3). |
| 存放说明 | -20C |
| 计算分子量 | 15 |
| 参考文献 | 1 . Frank, D. et al. (1999) Mamm Genome 10, 1150-9. 2 . Kerley-Hamilton, J.S. et al. (2005) Oncogene 24, 6090-100. 3 . Kawase, T. et al. (2009) Cell 136, 535-50. |
Western blot analysis of extracts from A172 and MCF7 cells using PHLDA3 Antibody. Western blot 检测A172和MCF7 细胞提取物，使用抗体为PHLDA3 Antibody。 |
