NF-κB1 p105 Antibody【科瑞奥博】

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NF-κB1 p105 Antibody

货号： 4717T 基本售价： 1350.0 元规格： -

产品信息

概述
货号	4717T
反应种属	Human,Mouse,Rat,Monkey,Mink,Bovine,Pig,
来源宿主	Rabbit
应用	WB, IP
目标/特异性	NF-κB1 p105 Antibody detects endogenous levels of total NF-κB1 p105 protein. It does not cross-react with the p50 subunit or other NF-κB family members.
使用方法	WB(1:1000) IP (1:50)

性能
供应商	CST
灵敏度	Endogenous
背景	Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11).
存放说明	-20C
计算分子量	120
参考文献	1 . Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79. 2 . Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20. 3 . Haskill, S. et al. (1991) Cell 65, 1281-9. 4 . Thompson, J.E. et al. (1995) Cell 80, 573-82. 5 . Whiteside, S.T. et al. (1997) EMBO J 16, 1413-26. 6 . Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83. 7 . Scherer, D.C. et al. (1995) Proc Natl Acad Sci USA 92, 11259-63. 8 . Chen, Z.J. et al. (1996) Cell 84, 853-62. 9 . Senftleben, U. et al. (2001) Science 293, 1495-9. 10 . Coope, H.J. et al. (2002) EMBO J 21, 5375-85. 11 . Xiao, G. et al. (2001) Mol Cell 7, 401-9. 12 . Heissmeyer, V. et al. (2001) Mol Cell Biol 21, 1024-35. 13 . Orian, A. et al. (2000) EMBO J 19, 2580-91.

参考图片

Western blot analysis of extracts from THP-1 cells, differentiated with TPA (#9905, 80 nM for 24 h) and treated with 1 μg/ml LPS for the indicated times, using NF-κB1 p105 Antibody.Western免疫印迹。用NF-κB1 p105 Antibody研究经TPA (#9905, 80 nM ,24 h)分化的然后用1 μg/ml LPS处理一定时间的THP-1细胞的细胞提取液。