| 货号 | 4738T |
| 反应种属 | Human,Monkey, |
| 来源宿主 | Rabbit |
| 应用 | WB |
| 目标/特异性 | ARP3 Antibody detects endogenous levels of total ARP3 protein. The antibody does not cross-react with endogenous levels of total ARP2 protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Actin nucleation, the formation of new actin filaments from existing filaments, affects actin filament structure during cell motility, division, and intracellular trafficking. An important actin nucleation protein complex is the highly conserved ARP2/3 complex, consisting of ARP2, ARP3 and ARPC1-5. The ARP2/3 complex promotes branching of an existing actin filament and formation of a daughter filament following activation by nucleation-promoting factors, such as WASP/WAVE or cortactin (1). The formation of podosomes, small cellular projections that degrade the extracellular matrix, is enhanced by ARP2/3 complex action. ARP2/3 competes with caldesmon, an actin binding protein shown to negatively affect podosome formation (2). Along with N-WASP, the ARP2/3 complex regulates nuclear actin filament nucleation and controls actin polymerization during transcription (3).肌动蛋白的核化是从已存在的微丝上形成新的微丝，在细胞运动、分裂和胞内运输中该核化影响微丝结构。一个重要肌动蛋白核化蛋白复合物是高度保守ARP2/3复合物，是由ARP2、ARP3和ARPC1-5组成。通过核化促进因子例如 WASP/WAVE或cortactin活化之后，ARP2/3复合物促进一个已存在微丝的分支和新微丝的形成(1)。伪足的形成是降解细胞外基质细胞的小的生理过程，其能通过ARP2/3复合物肌动蛋白提高。ARP2/3蛋白与钙结合蛋白竞争，钙结合蛋白是一个actin结合蛋白并显示负性调节伪足的形成(2)。连同N-WASP一起，ARP2/3 复合物调节细胞核微丝核化，以及在转录中控制actin聚合(3)。 |
| 存放说明 | -20C |
| 计算分子量 | 47 |
| 参考文献 | 1 . Goley, E.D. and Welch, M.D. (2006) Nat Rev Mol Cell Biol 7, 713-26. 2 . Morita, T. et al. (2007) J. Biol. Chem. 282, 8454-8463. 3 . Yoo, Y. et al. (2007) J. Biol. Chem. 282, 7616-7623. |
Western blot analysis of extracts from HeLa, Jurkat and COS cells using ARP3 Antibody.使用ARP3 Antibody，免疫印迹（Western Blot）分析HeLa、Jurkat和COS细胞系ARP3蛋白水平。 |
