| 货号 | 2010T |
| 反应种属 | Human, |
| 来源宿主 | Rabbit |
| 应用 | WB, IF-IC |
| 目标/特异性 | Atg12 Antibody (Human Specific) detects endogenous levels of total free and Atg5 bound Atg12 protein. |
| 使用方法 | WB(1:1000) IF-IC (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 16, 55 |
| 参考文献 | 1 . Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21. 2 . Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509-18. 3 . Levine, B. and Yuan, J. (2005) J Clin Invest 115, 2679-88. 4 . Mizushima, N. et al. (1998) J Biol Chem 273, 33889-92. 5 . Mizushima, N. et al. (1998) Nature 395, 395-8. 6 . Suzuki, K. et al. (2001) EMBO J 20, 5971-81. 7 . Tanida, I. et al. (1999) Mol Biol Cell 10, 1367-79. 8 . Shintani, T. et al. (1999) EMBO J 18, 5234-41. |
Western blot analysis of extracts from various cell lines using Atg12 Antibody (Human Specific). Western blot 分析多种细胞系的细胞提取物，使用抗体是Atg12 Antibody（人特异性的）。 | |
Confocal immunofluorescent analysis of HCT-116 cells, untreated (upper) or chloroquine-treated (lower), using Atg12 Antibody (Human Specific) (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光试验分析HCT-116细胞，未处理（上图）或氯喹处理（下图），使用抗体是Atg12 Antibody（人特异性的）（绿色）。蓝色伪彩=DRAQ5® #4084（荧光DNA染料）。 |
