| 货号 | 2002T |
| 反应种属 | Human, |
| 来源宿主 | Rabbit |
| 应用 | WB, IP |
| 目标/特异性 | BID Antibody (Human Specific) detects endogenous levels of both the full length (22 kDa) and cleaved large fragment (15 kDa) of human BID. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The BH3 domain-only protein, BID, a death agonist member of the Bcl-2/Bcl-xL family (1), is localized in the cytosolic fraction of cells as an inactive precursor (2,3). Its active form is generated upon proteolytic cleavage by caspase-8 in the Fas signaling pathway. Cleaved BID translocates to mitochondria and induces cytochrome c release and mitochondrial damage (2-5). Thus, BID relays an apoptotic signal from the cell surface to mitochondria. However, the precise molecular mechanism for the translocation of the cleaved BID, and for the subsequent release of cytochrome c during apoptosis, is still unclear. |
| 存放说明 | -20C |
| 计算分子量 | 15, 22 |
| 参考文献 | 1 . Wang, K. et al. (1996) Genes Dev 10, 2859-69. 2 . Luo, X. et al. (1998) Cell 94, 481-90. 3 . Li, H. et al. (1998) Cell 94, 491-501. 4 . Gross, A. et al. (1999) J Biol Chem 274, 1156-63. 5 . Li, P. et al. (1997) Cell 91, 479-489. 6 . Zou, H. et al. (1999) J. Biol. Chem. 274, 11549-11556. 7 . Saleh, A. et al. (1999) J Biol Chem 274, 17941-5. 8 . Yin, X.M. et al. (1999) Nature 400, 886-91. 9 . Korsmeyer, S.J. et al. (2000) Cell Death Differ 7, 1166-73. |
Western blot analysis of extracts from Jurkat cells, untreated or etoposide-treated (25 µM), and RS4;11 cells, untreated or okadaic acid-treated (1 µM), using BID Antibody (Human Specific). 采用 BID Antibody (Mouse Specific) 对未处理或 etoposide (依托泊苷)(25 µM）或 TNF-α-(20 ng/ml)处理的 L929 细胞进行免疫印迹分析（western blot）。 |
