Caspase-9 Antibody (Human Specific)【科瑞奥博】

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Caspase-9 Antibody (Human Specific)

货号： 9502T 基本售价： 1350.0 元规格： -

产品信息

概述
货号	9502T
反应种属	Human,
来源宿主	Rabbit
应用	WB, F
目标/特异性	Caspase-9 Antibody detects endogenous levels of full length caspase-9 (47 kDa) and large fragments of caspase-9. The antibody does not recognize other caspases.
使用方法	WB(1:1000) F (1:100)

性能
供应商	CST
灵敏度	Endogenous
背景	Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf 1. Apaf-1 mediated activation of caspase-9 involves instrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Additional cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10).
存放说明	-20C
计算分子量	35, 37, 47
参考文献	1 . Duan, H. et al. (1996) J. Biol. Chem. 271, 16720-16724. 2 . Srinivasula, S. M. et al. (1996) J. Biol. Chem. 271, 27099-27106. 3 . Liu, X. et al. (1996) Cell 86, 147-157. 4 . Li, P. et al. (1997) Cell 91, 479-489. 5 . Zou, H. et al. (1999) J. Biol. Chem. 274, 11549-11556. 6 . Srinivasula, S.M. et al. (1998) Mol Cell 1, 949-57. 7 . Deveraux, Q. L. et al. (1998) EMBO J. 17, 2215-2223. 8 . Slee, E. A. et al. (1999) J. Cell Biol. 144, 281-292. 9 . Sun, X.M. et al. (1999) J Biol Chem 274, 5053-60. 10 . MacFarlane, M. et al. (1997) J. Cell Biol. 137, 469-479.

参考图片

Flow cytometric analysis of Jurkat cells, untreated (blue) or etoposide-treated (green), using Caspase-9 Antibody (Human Specific) compared to a nonspecific negative control antibody (red).

流式细胞术分析:未处理（蓝色）或 etoposide (依托泊苷)处理的Jurkat 细胞，抗体采用Caspase-9 Antibody (人特异性)，并用非特异性抗体做阴性对照（红色）。

Western blot analysis of HeLa cells, untreated, staurosporine-treated (1 µM) or cytochrome c-treated (0.25 mg/ml), and Jurkat cells, untreated or cytochrome c-treated, using Caspase-9 Antibody (Human Specific).

Western blot分析未处理、staurosporine(星胞菌素)(1 µM) 或cytochrome(细胞色素C)(0.25 mg/ml)处理的HeLa 细胞，及未处理、或细胞色素C 处理的Jurkat细胞，抗体采用Caspase-9 Antibody (人特异性)。