| 货号 | 4933T |
| 反应种属 | Human, |
| 来源宿主 | Rabbit |
| 应用 | WB, IP |
| 目标/特异性 | Claudin-1 Antibody detects endogenous levels of total claudin-1 protein. Based on sequence similarity, the antibody may cross-react with claudin-2. |
| 使用方法 | WB(1:1000) IP (1:25) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Tight junctions, or zonula occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces. Tight junctions are composed of claudin and occludin proteins, which join the junctions to the cytoskeleton (1,2). The claudin family is composed of 23 integral membrane proteins, and their expression, which varies among tissue types, may determine both the strength and properties of the epithelial barrier. Alteration in claudin protein expression pattern is associated with several types of cancer (2,3). Claudin-1 is expressed primarily in keratinocytes (4) and normal mammary epithelial cells, but is absent or reduced in breast carcinomas and breast cancer cell lines (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 20 |
| 参考文献 | 1 . Shin, K. et al. (2006) Annu Rev Cell Dev Biol 22, 207-35. 2 . Oliveira, S.S. and Morgado-Díaz, J.A. (2007) Cell Mol Life Sci 64, 17-28. 3 . Hewitt, K.J. et al. (2006) BMC Cancer 6, 186. 4 . Brandner, J.M. et al. (2002) Eur J Cell Biol 81, 253-63. 5 . Krämer, F. et al. (2000) Hum Genet 107, 249-56. 6 . Swisshelm, K. et al. (1999) Gene 226, 285-95. |
Immunoprecipitation of claudin-1 from A431 cell extract using Claudin-1 Antibody. Western blot analysis was performed using the same antibody.使用Claudin-1抗体对A431细胞提取claudin-1进行免疫沉淀实验。western blot分析使用同样的抗体进行。 | |
Western blot analysis of extracts from A431 and ZR-75 cells using Claudin-1 Antibody.使用Claudin-1抗体对A431和ZR-75细胞提取物进行western blot分析。 |
