| 货号 | 4958T |
| 反应种属 | Human,Mouse, |
| 来源宿主 | Rabbit |
| 应用 | WB, IP , IHC-P |
| 目标/特异性 | SLP-76 Antibody detects endogenous levels of total SLP-76 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | SH2 domain-containing leukocyte protein of 76 kDa (SLP-76) is a hematopoietic adapter protein that is important in multiple biochemical signaling pathways and necessary for T cell development and activation (1). ZAP-70 phosphorylates SLP-76 and LAT as a result of TCR ligation. SLP-76 has amino-terminal tyrosine residues followed by a proline rich domain and a carboxy-terminal SH2 domain. Phosphorylation of Tyr113 and Tyr128 result in recruitment of the GEF Vav and the adapter protein Nck (2). TCR ligation also mediates an association between SLP-76 and Itk, which is accomplished in part via the proline rich domain of SLP-76 and the SH3 domain of ITK. Furthermore, the proline rich domain of SLP-76 binds to the SH3 domains of Grb2-like adapter Gads (3-4). In resting cells, SLP-76 is predominantly in the cytosol. Upon TCR ligation, SLP-76 translocates to the plasma membrane and promotes the assembly of a multi-protein signaling complex that includes Vav, Nck, Itk and PLCg1 (1). The expression of SLP-76 is tightly regulated: the protein is detected at very early stages of thymocyte development, increases as thymocyte maturation progresses, and is reduced as cells mature to CD4+ CD8+ double-positive thymocytes (5). |
| 存放说明 | -20C |
| 计算分子量 | 76 |
| 参考文献 | 1 . Clements, J.L. (2003) Immunol Rev 191, 211-9. 2 . Bubeck Wardenburg, J. et al. (1998) Immunity 9, 607-16. 3 . Bunnell, S.C. et al. (2000) J Biol Chem 275, 2219-30. 4 . Liu, S.K. et al. (1999) Curr Biol 9, 67-75. 5 . Clements, J.L. et al. (1998) J Immunol 161, 3880-9. |
Immunohistochemical analysis of paraffin-embedded human tonsil using SLP-76 Antibody.免疫组织化学染色分析石蜡包埋人结肠组织。所用抗体为SLP-76 Antibody。 | |
Immunohistochemical analysis of paraffin-embedded human colon using SLP-76 Antibody in the presence of control peptide (left) or antigen-specific peptide (right).免疫组织化学染色分析石蜡包埋人结肠组织。在对照多肽 (左图) 或抗原特异性多肽(右图)存在下，所用抗体为SLP-76 Antibody。 | |
Western blot analysis of extracts from Jurkat and BW5147cells using SLP-76 Antibody.Western免疫印迹。用SLP-76 Antibody研究Jurkat 和 BW5147细胞的细胞提取液。 |
