| 货号 | 5195T |
| 反应种属 | Human,Mouse, |
| 来源宿主 | Rabbit |
| 应用 | WB, IP , IF-IC |
| 目标/特异性 | Lipin 1 Antibody detects endogenous levels of total Lipin 1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Lipin 1 was identified as a nuclear protein required for adipose tissue development (1). The expression of Lipin 1 is induced during adipocyte differentiation (1). The abnormal development of adipose tissues caused by mutations in the lipin 1 gene results in lipodystrophy, a condition associated with low body fat, fatty liver, hypertriglyceridemia, and insulin resistance (1). Lipin 1 plays a role in lipid metabolism in various tissues and cell types including liver, muscle, adipose tissues, and neuronal cell lines (2-4). It has dual functions at the molecular level: Lipin 1 serves as a transcriptional coactivator in liver, and a phosphatidate phosphatase in triglyceride and phospholipid biosynthesis pathways (5). Lipin1 is regulated by mTOR, illustrating a connection between adipocyte development and nutrient-sensing pathways (6). It also mediates hepatic insulin signaling by TORC2/CRTC2 (7). |
| 存放说明 | -20C |
| 计算分子量 | 130 |
| 参考文献 | 1 . Péterfy, M. et al. (2001) Nat Genet 27, 121-4. 2 . Finck, B.N. et al. (2006) Cell Metab 4, 199-210. 3 . Phan, J. and Reue, K. (2005) Cell Metab 1, 73-83. 4 . Verheijen, M.H. et al. (2003) Genes Dev 17, 2450-64. 5 . Reue, K. and Zhang, P. (2008) FEBS Lett 582, 90-6. 6 . Huffman, T.A. et al. (2002) Proc Natl Acad Sci U S A 99, 1047-52. 7 . Ryu, D. et al. (2009) Cell Metab 9, 240-51. |
Western blot analysis of extracts from human white pre-adipocytes and human white adipocytes using Lipin 1 Antibody (upper) and β-Actin Antibody #4967 (lower). Wetern blot检测人白色脂肪前脂肪细胞提取物和人白色脂肪细胞提取物，所用抗体为Lipin 1 Antibody (上)和β-Actin Antibody #4967 (下)。 | |
Confocal immunofluorescent analysis of 3T3-L1 adipocytes using Lipin 1 Antibody (red). Lipid droplets were labeled with BODIPY® 493/503 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 激光共聚焦免疫荧光检测3T3-L1 细胞，所用抗体为Lipin 1 Antibody（红），脂质滴用BODIPY® 493/503 标记（绿）。蓝色伪彩为DNA荧光染料(对应产品号为DRAQ5® #4084) |
