| 货号 | 2104T |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Grp94 Antibody detects endogenous levels of total Grp94 protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Secretory proteins are synthesized on polysomes and translocated into the endoplasmic reticulum (ER). Inside ER, these proteins are often modified by disulfide bond formation, amino-linked glycosylation and folding. The ER contains a pool of molecular chaperones, including Grp94, to help proteins fold properly. Grp94 is a glucose-regulated protein (1) with sequence homology to Hsp90 (2). In addition to its role to help some secretory proteins fold to their correct conformation (3), studies suggest that Grp94 derived from cancer cells also induces anti-tumor immune responses in mouse tumor models (4, 5). One reason for this tumor immunogenicity is that Grp94 binds to the peptides from proteins in cancer cells and can therefore present these peptides as tumor antigens (6). Furthermore, Grp94 has also been shown to induce maturation of dendritic cells (7). Taken together, Grp94 functions both as a tumor-specific antigen and as an activator of antigen-presenting cells to elicit immunity to cancers (8). |
| 存放说明 | -20C |
| 计算分子量 | 100 |
| 参考文献 | 1 . Lee, A.S. et al. (1981) Proc. Natl. Acad. Sci. USA 78, 4922-4925. 2 . Sorger, P.K. and Pelham, H.R. (1987) J. Mol. Biol. 194, 341-344. 3 . Argon, Y. and Simen, B.B. (1999) Semin. Cell Dev. Biol. 10, 495-505. 4 . Blachere, N.E. et al. (1997) J. Exp. Med. 186, 1315-1322. 5 . Tamura, Y. et al. (1997) Science 278, 117-120. 6 . Schild, H. and Rammensee, H.G. (2000) Nat. Immunol. 1, 100-101. 7 . Singh-Jasuja, H. et al. (2000) Eur. J. Immunol. 30, 2211-2215. 8 . Nicchitta, C.V. et al. (2004) Cell Stress Chaperones 9, 325-331. |
Western blot analysis of extracts from various cell types using Grp94 Antibody. 对多个细胞系使用Grp94 Antibody进行Western blot分析。 |
