| 货号 | 2143T |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | Rab5 Antibody detects endogenous levels of total Rab5 protein. |
| 使用方法 | WB(1:1000) IF-IC (1:150) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Rab5 is a member of the Ras superfamily of small Rab GTPases. Rab5 is localized at the plasma membrane and early endosomes and functions as a key regulator of vesicular trafficking during early endocytosis (1). The conformational change between Rab5 GTP/GDP states is essential for its biological function as a rate limiting regulator at multiple steps during endocytosis (1,2). Rab5 exerts its function by interacting with several Rab5-specific effectors (1-3). These proteins form complexes with Rab5 on a specialized Rab domain of the endosome and promote recycling of Rab5-cargo targets between endosome and the plasma membrane. |
| 存放说明 | -20C |
| 计算分子量 | 25 |
| 参考文献 | 1 . Zerial, M. and McBride, H. (2001) Nat Rev Mol Cell Biol 2, 107-17. 2 . van der Bliek, A.M. (2005) Nat Cell Biol 7, 548-50. 3 . Haas, A.K. et al. (2005) Nat Cell Biol 7, 887-93. |
Confocal immunofluorescent analysis of MCF-7 cells using Rab5 Antibody (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). 对MCF-7细胞使用Rab5 Antibody（绿色）进行共聚焦免疫荧光分析。蓝色伪彩=DRAQ5®(荧光DNA染料)。 | |
Western blot analysis of extracts from MCF-7, Neuro2A, PC12 and COS cells, using Rab5 Antibody. 对MCF-7, Neuro2, PC12和CHO细胞裂解液使用Rab5 Antibody进行Western blot分析。 |
