| 货号 | 2202T |
| 反应种属 | Mouse |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Caspase-12 Antibody detects endogenous levels of full-length caspase-12 protein (55 kDa) and its cleaved product (42 kDa). The antibody does not cross-react with other caspases. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Caspase-12 is located in the endoplasmic reticulum (ER). It is responsible for ER-stress-induced apoptosis, such as high calcium concentration, low oxygen and low glucose levels (1,2). One of the mechanisms for caspase-12 activation is related to calpain mediated cleavage at T132 and K158, both of which are located at the amino-terminal region of the caspase-12 (2,3). Caspase-12 also has a putative caspase cleavage site located at the carboxy-terminal region of the protein (3). In cortical neurons, caspase-12 is involved at least partially in the amyloid-beta neurotoxicity process (1). |
| 存放说明 | -20C |
| 计算分子量 | 42, 55 |
| 参考文献 | 1 . Nakagawa, T. et al. (2000) Nature 403, 98-103. 2 . Nakagawa, T. and Yuan, J. (2000) J. Cell Biol. 150, 887-894. 3 . Van de Craen, M. et al. (1997) FEBS Lett. 403, 61-69. |
Western blot analysis of extracts from L929 cells, untreated or Brefeldin A-treated (10 µM, 30 hours), using Caspase-12 Antibody. Western blot 免疫印迹。用Caspase-12 Antibody研究未经处理和经Brefeldin A (10 µM, 30 小时)处理的L929细胞的细胞提取液。 |
