| 货号 | 5629T |
| 反应种属 | Human,Mouse, |
| 来源宿主 | Rabbit |
| 应用 | WB, IP |
| 目标/特异性 | RXRγ Antibody recognizes endogenous levels of total RXRγ protein. This antibody does not cross-react with either RXRα or RXRβ. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The human retinoid X receptors (RXRs) are encoded by three distinct genes (RXRα, RXRβ, and RXRγ) and bind selectively and with high affinity to the vitamin A derivative, 9-cis-retinoic acid. RXRs are type-II nuclear hormone receptors that are largely localized to the nuclear compartment independent of ligand binding. Nuclear RXRs form heterodimers with nuclear hormone receptor subfamily 1 proteins, including thyroid hormone receptor, retinoic acid receptors, vitamin D receptor, peroxisome proliferator-activated receptors, liver X receptors, and farnesoid X receptor (1). Since RXRs heterodimerize with multiple nuclear hormone receptors, they play a central role in transcriptional control of numerous hormonal signaling pathways by binding to cis-acting response elements in the promoter/enhancer region of target genes (2).人视黄醇X受体（RXRs）由三种不同的基因（RXRα，RXRβ，RXRγ）编码，并与具有高亲和力的维生素A衍生物，9-顺-视黄酸选择性结合。 RXRs是II型核激素受体，主要位于核室，与非依赖性配体结合。核RXR与核激素受体亚族蛋白形成异二聚体，包括甲状腺激素受体、维甲酸受体、维生素D受体、过氧化物酶体增殖物活化受体、肝脏X受体、法尼酯X受体（1）。由于RXR与多个核激素受体形成异源二聚体，通过与靶基因启动子/增强区域的顺式作用元件结合，在众多激素信号转导通路的转录调控中发挥核心作用（2）。 |
| 存放说明 | -20C |
| 计算分子量 | 55 |
| 参考文献 | 1 . Gronemeyer, H. et al. (2004) Nat Rev Drug Discov 3, 950-64. 2 . Mangelsdorf, D.J. et al. (1992) Genes Dev 6, 329-44. 3 . Krezel, W. et al. (1996) Proc Natl Acad Sci USA 93, 9010-4. 4 . Dollé, P. et al. (1994) Mech Dev 45, 91-104. 5 . Sugawara, A. et al. (1995) Endocrinology 136, 1766-74. 6 . Liu, Q. and Linney, E. (1993) Mol Endocrinol 7, 651-8. 7 . Georgiades, P. and Brickell, P.M. (1997) Dev Dyn 210, 227-35. 8 . Downes, M. et al. (1994) Endocrinology 134, 2658-61. 9 . Muscat, G.E. et al. (1994) Nucleic Acids Res 22, 583-91. 10 . Downes, M. et al. (1993) Cell Growth Differ 4, 901-9. 11 . Haugen, B.R. et al. (1997) Mol Endocrinol 11, 481-9. 12 . Brown, N.S. et al. (2000) J Clin Invest 106, 73-9. 13 . Sharma, V. et al. (2006) Endocrinology 147, 1438-51. |
Western blot analysis of extracts from 293T cells, either mock transfected or transfected with human RXRα, RXRβ, or RXRγ DYKDDDK-tagged constructs, using RXRγ Antibody (upper) and DYKDDDK Tag Antibody (Binds to same epitope as Sigmas Anti-FLAG® M2 Antibody) #2368 (lower).Western blot方法检测293T细胞提取物，细胞不转染或用DYKDDDDK标记的人RXRα, RXRβ或RXRγ构建体转染，使用的抗体为RXRγ Antibody (上图)和DYKDDDK Tag Antibody (与Sigma的 Anti-FLAG® M2 Antibody结合相同的抗原表位) #2368 (下图). | |
Western blot analysis of extracts from various tissues using RXRγ Antibody.Western blot方法检测多个组织提取物，使用的抗体为RXRγ Antibody. |
