| 货号 | 2782T |
| 反应种属 | Human, |
| 来源宿主 | Rabbit |
| 应用 | WB |
| 目标/特异性 | FADD Antibody (Human Specific) detects endogenous levels of human FADD protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Fas-associated death domain (FADD or Mort 1) functions as an important adaptor in coupling death signaling from membrane receptors, such as the Fas ligand and TNF family (DR3, DR4 and DR5), to caspase-8 (1,2). FADD has a carboxy-terminal death domain, which interacts with the cytoplasmic tail of the membrane receptor, and an amino-terminal death effector domain, which interacts with caspase-8. Clustering of the receptors upon stimulation brings about FADD and caspase-8 oligomerization, activating the caspase signaling pathway. Human FADD is phosphorylated mainly at Ser194, while mouse FADD is phosphorylated at Ser191. In both cases, the phosphorylation is cell cycle-dependent (3) and may be related to its regulatory role in embryonic development and cell cycle progression (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 28 |
| 参考文献 | 1 . Ashkenazi, A. and Dixit, V.M. (1998) Science 281, 1305-1308. 2 . Kuang, A. A. et al. (2000) J. Biol. Chem. 275, 25065-25068. 3 . Scaffidi, C. et al. (2000) J. Immunol. 164, 1236-1242. 4 . Newton, K. et al. (2000) EMBO J. 19, 931-941. 5 . Zhang, J. et al. (2001) J. Biol. Chem. 276, 29815-29818. |
Western blot analysis of extracts from Jurkat and HeLa cells treated with 4 mM hydroxyurea or 1 µg/ml nocodazole (each for 20 hours), using FADD Antibody (Human Specific). Western blot分析Jurkat和HeLa 细胞提取物，4 mM hydroxyurea(羟基脲)或1 µg/ml nocodazole 处理（每个20hr），使用抗体为FADD Antibody (Human Specific)。 |
