| 货号 | 2690T |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | IKKε Antibody detects endogenous levels of total IKKε protein. This antibody does not cross-react with other IKKs or with TBK1/NAK. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex, whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation; Ser177 and Ser181 in the activation loop of IKKβ (serine 176 and 180 in IKKα) are the specific sites whose phosphorylation causes conformational changes resulting in kinase activation (10-13). |
| 存放说明 | -20C |
| 计算分子量 | 80 |
| 参考文献 | 1 . Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6. 2 . Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70. 3 . Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8. 4 . Brown, K. et al. (1995) Science 267, 1485-8. 5 . Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18. 6 . Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83. 7 . Chen, Z.J. et al. (1996) Cell 84, 853-62. 8 . Zandi, E. et al. (1997) Cell 91, 243-52. 9 . Karin, M. (1999) Oncogene 18, 6867-74. 10 . DiDonato, J.A. et al. (1997) Nature 388, 548-54. 11 . Mercurio, F. et al. (1997) Science 278, 860-6. 12 . Johnson, L.N. et al. (1996) Cell 85, 149-58. 13 . Delhase, M. et al. (1999) Science 284, 309-13. 14 . Tojima, Y. et al. (2000) Nature 404, 778-82. 15 . Bonnard, M. et al. (2000) EMBO J 19, 4976-85. 16 . Shimada, T. et al. (1999) Int Immunol 11, 1357-62. 17 . Peters, R.T. et al. (2000) Mol Cell 5, 513-22. 18 . Peters, R.T. and Maniatis, T. (2001) Biochim Biophys Acta 1471, M57-62. |
Western blot analysis of recombinant IKKε Kinase #7553 and RAW 264.7 cells, untreated or LPS-treated, using IKKε Antibody.Western免疫印迹。用IKKε Antibody研究重组的IKKε Kinase #7553 和未经处理的和经LPS处理的RAW 264.7 细胞的细胞提取液。 |
