| 货号 | 8715T |
| 反应种属 | Human,Mouse, |
| 来源宿主 | Rabbit |
| 应用 | WB, IP |
| 目标/特异性 | RXRβ Antibody recognizes endogenous levels of total RXRβ protein. This antibody does not cross-react with either RXRα or RXRγ proteins. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The human retinoid X receptors (RXRs) are encoded by three distinct genes (RXRα, RXRβ, and RXRγ) and bind selectively and with high affinity to the vitamin A derivative, 9-cis-retinoic acid. RXRs are type-II nuclear hormone receptors that are largely localized to the nuclear compartment independent of ligand binding. Nuclear RXRs form heterodimers with nuclear hormone receptor subfamily 1 proteins, including thyroid hormone receptor, retinoic acid receptors, vitamin D receptor, peroxisome proliferator-activated receptors, liver X receptors, and farnesoid X receptor (1). Since RXRs heterodimerize with multiple nuclear hormone receptors, they play a central role in transcriptional control of numerous hormonal signaling pathways by binding to cis-acting response elements in the promoter/enhancer region of target genes (2).人视黄醇X受体（RXRs）由三种不同的基因（RXRα，RXRβ，RXRγ）编码，并与具有高亲和力的维生素A衍生物，9-顺-视黄酸选择性结合。 RXRs是II型核激素受体，主要位于核室，非依赖性配体结合。核RXR与核激素受体亚家族蛋白形成异二聚体，包括甲状腺激素受体、维甲酸受体、维生素D受体、过氧化物酶体增殖物激活受体、肝脏X受体、法尼酯X受体（1）。由于RXR与多个核激素受体形成异源二聚体，通过与靶基因启动子/增强区域的顺式作用元件结合，在众多激素信号转导通路的转录调控中发挥核心作用（2）。 |
| 存放说明 | -20C |
| 计算分子量 | 70-72 |
| 参考文献 | 1 . Gronemeyer, H. et al. (2004) Nat Rev Drug Discov 3, 950-64. 2 . Mangelsdorf, D.J. et al. (1992) Genes Dev 6, 329-44. 3 . Nagata, T. et al. (1994) Gene 142, 183-9. 4 . Fleischhauer, K. et al. (1993) Hum Genet 90, 505-10. 5 . Fleischhauer, K. et al. (1992) Nucleic Acids Res 20, 1801. 6 . Hamada, K. et al. (1989) Proc Natl Acad Sci USA 86, 8289-93. 7 . Kastner, P. et al. (1996) Genes Dev 10, 80-92. 8 . Mascrez, B. et al. (2004) EMBO Rep 5, 285-90. |
Western blot analysis of extracts from 293T cells, either mock-transfected or transfected with a Myc/DDK-tagged cDNA expression construct encoding full-length human RXRα, RXRβ, and RXRγ, using RXRβ Antibody (upper) or DYKDDDDK Tag Antibody (Binds to same epitope as Sigmas Anti-FLAG® M2 Antibody) #2368 (lower).Western blot方法检测293T细胞提取物，细胞不转染或用Myc/DDK标记的cDNA表达构建体转染，该构建体编码人全长RXRα, RXRβ, and RXRγ，使用的抗体为RXRβ Antibody (上图)或DYKDDDDK Tag Antibody (与Sigma的Anti-FLAG® M2 Antibody结合相同的抗原表位) #2368 (下图)。 | |
Western blot analysis of extracts from various cell lines using RXRβ Antibody.Western blot方法检测多个细胞系的提取物，使用的抗体为RXRβ Antibody. |
