| 货号 | 9166T |
| 反应种属 | Human,Mouse, |
| 来源宿主 | Rabbit |
| 应用 | WB, IHC-P , F |
| 目标/特异性 | LAT Antibody detects endogenous levels of total LAT protein. |
| 使用方法 | WB(1:1000) IHC-P (1:100) F (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | LAT, a transmembrane adaptor protein expressed in T, NK and mast cells, is an important mediator for T cell receptor (TCR) signaling (1). Upon TCR engagement, activated Zap-70 phosphorylates LAT at multiple conserved tyrosine residues within SH2 binding motifs, exposing these motifs as the docking sites for downstream signaling targets (2,3). The phosphorylation of LAT at Tyr171 and Tyr191 enables the binding of Grb2, Gads/SLP-76, PLCγ1 and PI3 kinase through their SH2 domain and translocates them to the membrane. This process eventually leads to activation of the corresponding signaling pathways (1-4). |
| 存放说明 | -20C |
| 计算分子量 | 36, 38 |
| 参考文献 | 1 . Wonerow, P. and Watson, S.P. (2001) Oncogene 20, 6273-83. 2 . Zhang, W. et al. (1998) Cell 92, 83-92. 3 . Paz, P.E. et al. (2001) Biochem J 356, 461-71. 4 . Zhang, W. et al. (2000) J Biol Chem 275, 23355-61. |
Immunohistochemical analysis of paraffin-embedded human tonsil, showing membrane localization, using LAT Antibody.免疫组织化学染色分析石蜡包埋人扁桃腺组织,图片展示了其细胞膜的定位。所用抗体为LAT Antibody。 | |
Western blot analysis of extracts from Jurkat cells, untreated or treated with lambda phosphatase, using LAT Antibody.Western免疫印迹。用LAT Antibody研究未经处理或经过lambda磷酸酶处理的Jurkat细胞的细胞提取液。 | |
Flow cytometric analysis of Jurkat cells, using LAT Antibody (blue) compared to a nonspecific negative control antibody (red).流式细胞仪研究Jurkat细胞,所用抗体为LAT Antibody(蓝色)和非特异性的阴性对照抗体(红色)。 | |
Immunohistochemical analysis of paraffin-embedded Jurkat cells, using LAT Antibody in the presence of control peptide (left) or antigen-specific peptide (right).免疫组织化学染色分析石蜡包埋Jurkat 细胞, 在对照多肽(左图)或抗原特异性多肽(右图)存在下用抗体LAT Antibody进行研究。 |
