| 货号 | 9252T |
| 反应种属 | Human,Mouse,Rat,Hamster,Monkey,Zebrafish,Bovine,S. cerevisiae, |
| 来源宿主 | Rabbit |
| 应用 | WB |
| 目标/特异性 | SAPK/JNK Antibody detects endogenous levels of total JNK1, JNK2 or JNK3 protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, by growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germinal center kinase (GCK) family member (4). There are three SAPK/JNK genes each of which undergoes alternative splicing resulting in numerous isoforms (3). SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors (3,5). |
| 存放说明 | -20C |
| 计算分子量 | 46, 54 |
| 参考文献 | 1 . Davis, R.J. (1999) Biochem Soc Symp 64, 1-12. 2 . Ichijo, H. (1999) Oncogene 18, 6087-93. 3 . Kyriakis, J.M. and Avruch, J. (2001) Physiol Rev 81, 807-69. 4 . Kyriakis, J.M. (1999) J Biol Chem 274, 5259-62. 5 . Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62. 6 . Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem. Sci. 23, 481-485. |
Western blot analysis of extracts from 293 and SK-N-MC cells, untreated or UV-treated (40 J/m2), using Phospho-SAPK/JNK Antibody #9251 (upper) or SAPK/JNK Antibody (lower).western blot方法检测细胞提取物：未处理的和紫外照射(40 J/m2),的293 和SK-N-MC 细胞。使用的抗体是Phospho-SAPK/JNK Antibody #9251 (上图)和SAPK/JNK Antibody (下图)。 |
