| 货号 | 2854T |
| 反应种属 | Human, |
| 来源宿主 | Rabbit |
| 应用 | WB, IP |
| 目标/特异性 | XLF Antibody detects endogenous levels of total XLF protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Double stranded DNA breaks (DSB’s) are the most toxic of DNA lesions. They occur in response to genotoxic stress, and they are also an obligate intermediate in the V(D)J recombination events in the immune system. The mechanism by which cells deal with DSB’s is known as NHEJ (non-homologous end-joining), and involves a core group of proteins that includes Ku, DNA-PK, XRCC4, and XLF (1). XLF, also known as Cernunnos, was originally discovered as a mutated protein from cells of individuals who displayed features of growth retardation, microcephaly, and immunodeficiency (2). These cells were sensitive to ionizing radiation and defective in V(D)J recombination. Exogenous expression of wild type XLF corrected these deficiencies (3), indicating that XLF is a critical component of the NHEJ response. XLF physically interacts with and may stimulate the ligase activity of XRCC4 (3).双链DNA 断裂(DSB’s)是毒性最大的DNA损伤。其发生响应于基因毒性应激，它也是免疫系统中V(D)J重组事件必需的中间态。细胞处理DSB’s的机制被称作NHEJ (非同源末端连接)，它涉及一组核心的蛋白，包括Ku, DNA-PK, XRCC4和XLF (1)。XLF，亦被称作Cernunnos，最初作为一种突变蛋白发现于以生长迟缓、小颅和免疫缺陷为特征的个体细胞中(2)。这些细胞对离子辐射敏感且存在V(D)J重组缺陷。野生型XLF的外源表达可以纠正这些缺陷(3)，表明XLF是NHEJ反应的关键组成部分。XLF生理上可以与XRCC4相互作用并能促进其连接酶活性(3)。 |
| 存放说明 | -20C |
| 计算分子量 | 39 |
| 参考文献 | 1 . Tsai, C.J. et al. (2007) Proc Natl Acad Sci U S A 104, 7851-6. 2 . Buck, D. et al. (2006) Cell 124, 287-99. 3 . Ahnesorg, P. et al. (2006) Cell 124, 301-13. |
Western blot analysis of extracts of various cell lines using XLF Antibody.western blot方法检测不同细胞提取物，检测抗体为XLF Antibody。 |
