| 货号 | 2589T |
| 反应种属 | Human, |
| 来源宿主 | Rabbit |
| 应用 | WB, IP |
| 目标/特异性 | DLL4 Antibody detects endogenous levels of total DLL4 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Notch signaling is activated upon engagement of the Notch receptor with its ligands, the DSL (Delta, Serrate, Lag2) proteins of single-pass type I membrane proteins. The DSL proteins contain multiple EGF-like repeats and a DSL domain that is required for binding to Notch (1,2). Five DSL proteins have been identified in mammals: Jagged1, Jagged2, Delta-like (DLL) 1, 3 and 4 (3). Ligand binding to the Notch receptor results in two sequential proteolytic cleavages of the receptor by the ADAM protease and the γ-secretase complex. The intracellular domain of Notch is released and then translocates to the nucleus where it activates transcription. Notch ligands may also be processed in a way similiar to Notch, suggesting a bi-directional signaling through receptor-ligand interactions (4-6). |
| 存放说明 | -20C |
| 计算分子量 | 75-80 |
| 参考文献 | 1 . Wilson, A. and Radtke, F. (2006) FEBS Lett. 580, 2860-2868. 2 . Hansson, E.M. et al. (2004) Semin. Cancer Biol. 14, 320-328. 3 . Chiba, S. (2006) Stem Cells 24, 2437-2447. 4 . Bland, C.E. et al. (2003) J. Biol. Chem. 278, 13607-13610. 5 . Six, E. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 7638-7643. 6 . LaVoie, M.J. and Selkoe, D.J. (2003) J. Biol. Chem. 278, 34427-34437. 7 . Shutter, J.R. et al. (2000) Genes Dev 14, 1313-8. 8 . Gale, N.W. et al. (2004) Proc Natl Acad Sci U S A 101, 15949-54. 9 . Krebs, L.T. et al. (2004) Genes Dev 18, 2469-73. 10 . Duarte, A. et al. (2004) Genes Dev 18, 2474-8. 11 . Hellström, M. et al. (2007) Nature 445, 776-80. 12 . Noguera-Troise, I. et al. (2006) Nature 444, 1032-7. 13 . Lobov, I.B. et al. (2007) Proc Natl Acad Sci U S A 104, 3219-24. 14 . Scehnet, J.S. et al. (2007) Blood 109, 4753-60. |
Western blot analysis of extracts from HUVEC and COS cells, untransfected or transiently transfected with a construct expressing human DLL4, using DLL4 Antibody. 使用DLL4抗体对未转染或瞬转了人DLL4表达质粒的HUVEC和COS细胞提取物进行western blot分析。 |
