| 货号 | 3682T |
| 反应种属 | Human,Mouse,Rat,Monkey,Bovine, |
| 来源宿主 | Rabbit |
| 应用 | WB, IP |
| 目标/特异性 | Mst1 Antibody detects endogenous levels of total Mst1 protein. The antibody does not cross-react with Mst2-4. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Mst kinases, members of the STE20 family of kinases, are upstream activators of MAPK pathways that regulate processes such as apoptosis, morphogenesis and cytoskeletal rearrangements. The amino-terminal kinase domain of Mst is considerably homologous to the kinase domain of yeast STE20 kinase and other p21-activated kinases (1). The carboxy-terminal region of Mst1 and Mst2 contains dimerization and inhibitory domains (1-3). Dimerization and phosphorylation at the activation loop results in translocation of Mst1 from the cytosol to the nucleus (3). Growing evidence indicates that Mst1, Mst2 and Mst3 are activated by apoptotic signals as well as other stress conditions (4-6). Complete activation of Mst1 requires both phosphorylation and caspase-mediated cleavage (4). Sequence alignment of the activation loop of the GCK family indicates that Thr183 of Mst1 and Thr180 of Mst2 are the conserved residues and might be critical for the activity of the kinases. Activated Mst kinases may rely on p38 MAPK and JNK pathways to amplify apoptotic signals (5). Phosphorylation at Ser327 of Mst1, which is close to the caspase-3 recognition site, inhibits caspase-mediated cleavage (4). |
| 存放说明 | -20C |
| 计算分子量 | 59 |
| 参考文献 | 1 . Dan, I. et al. (2001) Trends Cell Biol 11, 220-30. 2 . Creasy, C.L. et al. (1996) J Biol Chem 271, 21049-53. 3 . Lee KK and Yonehara S (2002) J Biol Chem 277, 12351–8 4 . Ura, S. et al. (2001) Proc Natl Acad Sci U S A 98, 10148-53. 5 . Zhao, B. et al. (2011) Nat Cell Biol 13, 877-83. |
Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells, using Mst1 Antibody. 使用Mst1 Antibody，免疫印迹（Western Blot）分析HeLa、NIH/3T3、C6和COS细胞中Mst1蛋白水平。 |
