| 货号 | 3415T |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit |
| 应用 | WB |
| 目标/特异性 | Atg3 Antibody detects endogenous levels of total Atg3 protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related genes (Atg). Formation of the autophagic vesicles involves two ubiquitin-like conjugation systems, Atg12-Atg5 and Atg8-phosphatidylethanolamine (Atg8-PE), which are essential for autophagy and widely conserved in eukaryotes (2). There are at least three Atg8 homologs in mammalian cells, GATE-16, GABARAP, and LC3, that are conjugated by lipids (3,4). Lipid conjugation of Atg8 and its mammalian homologs requires Atg3 (Apg3p/Aut1p in yeast), an ubiquitously expressed E2-like enzyme (5-7). Following C-terminal cleavage by the cysteine protease Atg4, the exposed glycine residue of Atg8 binds to the E1-like enzyme Atg7, is transferred to Atg3, and then conjugated to phophatidylethanolamine. Atg3-deficient mice die within 1 day after birth and are completely defective for the conjugation of Atg8 homlogs and autophagome formation (8). |
| 存放说明 | -20C |
| 计算分子量 | 40 |
| 参考文献 | 1 . Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21. 2 . Ohsumi, Y. (2001) Nat Rev Mol Cell Biol 2, 211-6. 3 . Kabeya, Y. et al. (2000) EMBO J. 19, 5720-28. 4 . Kabeya, Y. et al. (2004) J. Cell Sci. 117, 2805-12. 5 . Tanida, I. et al. (2002) J Biol Chem 277, 13739-44. 6 . Ichimura, Y. et al. (2000) Nature 408, 488-92. 7 . Schlumpberger, M. et al. (1997) J Bacteriol 179, 1068-76. 8 . Sou, Y.S. et al. (2008) Mol Biol Cell 19, 4762-75. |
Western blot analysis of extracts from various cell lines using Atg3 Antibody. Western blot 分析多种细胞系的细胞提取物，使用抗体是Atg3 Antibody。 | |
Western blot analysis of extracts from HeLa cells, mock transfected or transfected with mouse Atg3, using Atg3 Antibody. Western blot 分析HeLa细胞的细胞提取物，对照转染或转染鼠源Atg3，使用抗体为Atg3 Antibody。 |
