Caspase-9 Antibody (Mouse Specific)【科瑞奥博】

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Caspase-9 Antibody (Mouse Specific)

货号： 9504T 基本售价： 1350.0 元规格： -

产品信息

概述
货号	9504T
反应种属	Mouse,
来源宿主	Rabbit
应用	WB
目标/特异性	Caspase-9 Antibody (Mouse Specific) detects endogenous levels of both full length mouse caspase-9 (49 kDa) and the large fragment of mouse caspase-9 resulting from cleavage at aspartic acid 353 (37 kDa) and/or aspartic acid 368 (39 kDa). The antibody does not cross-react other caspases.
使用方法	WB(1:1000)

性能
供应商	CST
灵敏度	Endogenous
背景	Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf 1. Apaf-1 mediated activation of caspase-9 involves instrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Additional cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10).
存放说明	-20C
计算分子量	37, 39, 49
参考文献	1 . Duan, H. et al. (1996) J. Biol. Chem. 271, 16720-16724. 2 . Srinivasula, S. M. et al. (1996) J. Biol. Chem. 271, 27099-27106. 3 . Liu, X. et al. (1996) Cell 86, 147-157. 4 . Li, P. et al. (1997) Cell 91, 479-489. 5 . Zou, H. et al. (1999) J. Biol. Chem. 274, 11549-11556. 6 . Srinivasula, S.M. et al. (1998) Mol Cell 1, 949-57. 7 . Deveraux, Q. L. et al. (1998) EMBO J. 17, 2215-2223. 8 . Slee, E. A. et al. (1999) J. Cell Biol. 144, 281-292. 9 . Sun, X.M. et al. (1999) J Biol Chem 274, 5053-60. 10 . MacFarlane, M. et al. (1997) J. Cell Biol. 137, 469-479.

参考图片

Western blot analysis of extracts from NIH/3T3 cells, untreated, staurosporine-treated (1 µM), or cytochrome c-treated (0.25 mg/ml), using Caspase-9 Antibody. p39: caspase-9 cleaved at Asp368. p37: caspase-9 cleaved at Asp353.

Western blot分析未处理、staurosporine(星胞菌素)(1 µM) 或 cytochrome(细胞色素C)(0.25 mg/ml)处理的NIH/3T3 细胞，抗体采用Caspase-9 Antibody p39: caspase-9 Asp368处裂解获得. p37: caspase-9 Asp353处裂解获得。