| 货号 | 2490T |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | eIF4A1 Antibody detects endogenous levels of total eIF4A1 protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | A variety of factors contribute to the important biological event of initiation of translation. The eIF4F complex of translation initiation factors binds to the 5 m7 GTP cap to open up the mRNA secondary structure and allow small ribosome subunit binding (1). eIF4A, an eIF4 complex component that acts as an ATP-dependent RNA helicase, unwinds the secondary structure of the 5 mRNA untranslated region to mediate ribosome binding (2,3). In addition, eIF4A has recently been shown to repress Dpp/BMP signalling in a translation-independent manner in Drosophila (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 48 |
| 参考文献 | 1 . Rogers, G.W. et al. (2001) J Biol Chem 276, 12598-608. 2 . Rogers, G.W. et al. (1999) J Biol Chem 274, 12236-44. 3 . Svitkin, Y.V. et al. (2001) RNA 7, 382-94. 4 . Li, J. and Li, W.X. (2006) Nat. Cell Biol. 8, 1407-1414. 5 . Affolter, M. and Pyrowolakis, G. (2006) Nat. Cell Biol. 8, 1319-1321. |
Confocal immunofluorescent analysis of HeLa cells using eIF4A1 Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). 使用eIF4A1 Antibody（绿色）标记，共聚焦免疫荧光观察eIF4A1蛋白在HeLa细胞定位。Alexa Fluor® 555 phalloidin标记微丝蛋白（红色）。蓝色伪彩 = DRAQ5™ (DNA 荧光染料)。 | |
Western blot analysis of extracts from various cell lines, using eIF4A1 Antibody. 使用eIF4A1 Antibody，免疫印迹（Western Blotting）分析不同细胞系提取物。 |
