| 货号 | 2368T |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | DYKDDDDK Tag Antibody detects exogenously expressed DYKDDDDK proteins in cells. The antibody recognizes the DYKDDDDK peptide (the same epitope recognized by Sigmas Anti-FLAG® antibodies) fused to either the amino- or carboxy-terminus of targeted proteins. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:200) |
| 供应商 | CST |
| 灵敏度 | Transfected Only |
| 背景 | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged proteins biochemical properties. |
| 存放说明 | -20C |
| 参考文献 | 1 . Brizzard, B.L. et al. (1994) Biotechniques 16, 730-5. |
Western blot analysis of extracts from COS or 293T cells, mock transfected (lane 1) or expressing DYKDDDDK-tagged p73 (lane2), DYKDDDDK-tagged FKHRL1 (lane 3) or truncated DYKDDDDK-tagged huntingtin (lane 4), using DYKDDDDK Tag Antibody.Western blot 分析COS和293T细胞的提取物，泳道1为mock转染组，泳道2为采用DYKDDDDK--tagged p73转染组，泳道3为DYKDDDDK-tagged FKHRL1 转染组，泳道4为truncated DYKDDDDK-tagged huntingtin转染组。所用抗体为DYKDDDDK Tag Antibody 。 | |
Flow cytometric analysis of DYKDDDDK Tag Rabbit antibody in Cos cells transfected with a Myc-DDK-tagged SUMF1 construct and gated on transfected (green) or untransfected (blue) cells using Myc-Tag (9B11) Mouse mAb (Alexa Fluor® Conjugate) #2279. |
