| 货号 | 2365T |
| 反应种属 | All Species Expected, |
| 来源宿主 | Rabbit |
| 应用 | WB, IP |
| 目标/特异性 | His-Tag Antibody detects recombinant proteins containing the 6xHis epitope tag. The antibody recognizes the 6xHis-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. |
| 使用方法 | WB(1:1000) IP (1:25) |
| 供应商 | CST |
| 灵敏度 | Transfected Only |
| 背景 | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged proteins biochemical properties. |
| 存放说明 | -20C |
| 参考文献 | 1 . Kroll, D.J. et al. (1993) DNA Cell Biol 12, 441-53. 2 . di Guan, C. et al. (1988) Gene 67, 21-30. |
Western blot analysis of extracts from cells expressing C-terminal His-tagged protein (lane 1) or control extract (lane 2), using His-Tag Antibody.Weatern blot分析羧基末端表达His标签的蛋白（泳道1）和对照组提取物（泳道2），所用抗体为His-Tag Polyclonal Antibody。 | |
Immunoprecipitation of C-terminal His-tagged protein (lane 1) or N-terminal HA-tagged protein (lane 2), using His-Tag Antibody, then western analysis with the same antibody.免疫沉淀分析羧基末端表达His标签的蛋白（泳道1）和氨基末端表达His标签的蛋白（泳道2），所用抗体为His-Tag Polyclonal Antibody，采用相同抗体进行western分析 | |
Confocal immunofluorescent analysis of COS cells transfected with a His-tagged protein using His-Tag Polyclonal Antibody. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦免疫荧光分析转染His标签蛋白的COS细胞，所用抗体为His-Tag Polyclonal Antibody，蓝色伪彩为荧光DNA燃料，产品信息为DRAQ5 ® #4084。 |
